Journal: Frontiers in Nutrition

Article Title: Hydrogen-rich water improves endurance by reducing skeletal muscle oxidative stress and inflammatory responses

doi: 10.3389/fnut.2026.1722091

Figure Lengend Snippet: Baseline morphological and physiological characteristics of the mice following HRW intake. (A) Body weight (g) of the mice consuming purified water (PW) or hydrogen-rich water (HRW) (PW: n = 12 per group, HRW: n = 14 per group). (B) Forelimb grip strength (g/g), measured weekly during the intervention period (PW: n = 12 per group, HRW: n = 14 per group). (C) Wet masses of six hindlimb skeletal muscles (soleus, extensor digitorum longus (EDL), plantaris, tibialis cranialis (TC), gastrocnemius, and quadriceps femoris) (PW: n = 12 per group, HRW: n = 14 per group). (D) Representative images of gastrocnemius muscle cross-sections that were hematoxylin and eosin (HE)-stained or fluorescence immunostained (IF) for myosin heavy chain isoforms ( n = 3 per group).

Article Snippet: Primary antibodies against myosin heavy chain isoforms were applied overnight at 4 °C (type I (BA-D5), IIa (SC-71), IIx (6H1), or IIb (BF-F3) (all from DSHB, Iowa City, IA, USA)).

Techniques: Purification, Muscles, Staining, Fluorescence

Journal: Development (Cambridge, England)

Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

doi: 10.1242/dev.205047

Figure Lengend Snippet: Subsets of tenocytes are marked by active Wnt signaling during development. (A,F,K) Ventral views of cranial tendons at 48 hpf (A), 53 hpf (F) and 72 hpf (K) in Tg(scxa:mCherry; 7xTCF:GFP) zebrafish embryos immunostained for anti-mCherry (tenocytes, magenta), anti-GFP (Wnt responsive cells, green) and anti-MHC (muscle fibers, blue). (P) Ventral view of is HCR-stained Tg(7xTCF:GFP) 72 hpf embryo showing expression of scxa in tenocytes (magenta), sox9a in cartilage (blue) and co-immunostained with anti-GFP (Wnt responsive, green). (B-D,G-I,L-N,Q-S) Magnified views of region marked by white dotted lines in A,F,K,P, respectively, showing immunofluorescent signal of mCherry and GFP and is HCR expression of scxa , sox9a and IF staining of GFP. (E,J,O,T) Monochrome panel showing tenocytes co-expressing mCherry and GFP, and scxa and GFP. (U-W) Magnified view of region marked by red dotted lines in P showing is HCR expression of scxa , sox9a and IF staining of GFP. (X) Monochrome image showing tenocytes co-expressing scxa and GFP. Scale bars: 20 μm (A,F,K,Q,U); 30 μm (P); 15 μm (G); 10 μm (B,L).

Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

Techniques: Staining, Expressing

Journal: Development (Cambridge, England)

Article Title: Analysis of cranial tenocyte heterogeneity reveals a role for Wnt signaling in tendon attachments

doi: 10.1242/dev.205047

Figure Lengend Snippet: Genetic perturbation of Wnt signaling disrupts muscle attachments and tendon pattern. (A-R) Ventral views of cranial tendons at 48 hpf and 60 hpf Tg(scxa:mCherry) , Tg(scxa:mCherry; hsp70l:dkk1b-GFP) and Tg(scxa:mCherry; hsp70l:dnTCF-GFP) embryos that were heat shocked and immunostained for anti-mCherry (tenocytes), anti-GFP (Wnt responsive cells) and anti-MHC (muscle fibers). Images show muscle attachments in untreated (A,J) and heat-shocked controls (B,K), Tg(scxa:mCherry) and heat-shocked transgenics with perturbed Wnt signaling (D-I, M-R). (C,L) Histograms show distribution of normal, mild and severe muscle attachment defects between control and heat-shocked Tg(scxa:mCherry; hsp70l:dkk1b-GFP) embryos. P -values were calculated using chi-square test of independence. *** P <0.001 (48 hpf, n ≈79; 60 hpf, n ≈76). Scale bars: 30 μm.

Article Snippet: Embryos were stained using mouse monoclonal anti-Myosin heavy chain (MHC) antibody (Developmental Studies Hybridoma Bank, A4.1025; RRID:AB_528356) at 1:200 dilution, rat monoclonal anti-mCherry antibody (Invitrogen, M11217 ; RRID:AB_2536611) at 1:500 dilution, rabbit polyclonal anti p-Myosin light chain (p-MLC) antibody (Cell Signaling Technology, 3671; RRID:AB_330248) at 1:500 dilution, rabbit anti-Thrombospondin4b antibody (Thbs4b) (GeneTex, GTX125869; RRID:AB_2885605) at 1:1000 dilution.

Techniques: Control