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imp1088 (MedChemExpress)

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MedChemExpress imp1088
Imp1088, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imp1088/product/MedChemExpress
Average 94 stars, based on 8 article reviews

imp1088 - by Bioz Stars, 2026-05

94/100 stars

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(A-C) A549 cells were infected with YFV 17D, MOPV (A), LASV (B), or MACV (C) at MOI 0.01 for 1 h. Before, during and after infection, cells were treated or not with 10 fold dilutions of IMP1088, DDD85646 or C75 ranging from 1 nM to 10 μM. After 48 h of infection, cell culture supernatants were titrated (left panels) while cell viability was measured (right panels). The results of three independent experiments are represented as mean +/- SEM and expressed as FFU/mL for titrations and standardized to mock condition for viability. (D) Measure of the potential virucidal effect of IMP1088, DDD85646 and C75 on MOPV particles. 10 5 FFU of MOPV were incubated for 1 h with 10 μM of each compounds and were then titrated. The results of three independent experiments are represented as mean +/- SEM and expressed as FFU/mL. (E) Potency of IMP1088 and DDD85646 against MOPV, LASV, LCMV, LUJV, MACV or JUNV. A549 cells were infected (MOI 0.01) and treated or not with IMP1088 (20 nM), DDD85646 or C75 (500 nM) as described above. Cell culture supernatant were titrated and results of three independent experiments expressed as mean percentage +/- SEM to mock treated condition. P <0.05 (*) and <0.001 (***).

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: (A-C) A549 cells were infected with YFV 17D, MOPV (A), LASV (B), or MACV (C) at MOI 0.01 for 1 h. Before, during and after infection, cells were treated or not with 10 fold dilutions of IMP1088, DDD85646 or C75 ranging from 1 nM to 10 μM. After 48 h of infection, cell culture supernatants were titrated (left panels) while cell viability was measured (right panels). The results of three independent experiments are represented as mean +/- SEM and expressed as FFU/mL for titrations and standardized to mock condition for viability. (D) Measure of the potential virucidal effect of IMP1088, DDD85646 and C75 on MOPV particles. 10 5 FFU of MOPV were incubated for 1 h with 10 μM of each compounds and were then titrated. The results of three independent experiments are represented as mean +/- SEM and expressed as FFU/mL. (E) Potency of IMP1088 and DDD85646 against MOPV, LASV, LCMV, LUJV, MACV or JUNV. A549 cells were infected (MOI 0.01) and treated or not with IMP1088 (20 nM), DDD85646 or C75 (500 nM) as described above. Cell culture supernatant were titrated and results of three independent experiments expressed as mean percentage +/- SEM to mock treated condition. P <0.05 (*) and <0.001 (***).

Article Snippet: The IMP1088, 5-[3,4-difluoro-2-[2-(1,3,5-trimethyl-1H-pyrazol-4-yl)ethoxyphenyl]-N,N,1-trimethyl-1H-indazole-3-methanamine (CAS 2059148-82-0), DDD85646, 2,6-dichloro-4-[2-(1-piperazinyl)-4-pyridinyl]-N-(1,3,5-trimethyl-1H-pyrazol-4-yl)-benzenesulfonamide (CAS 1215010-55-1) and C75, tetrahydro-4-methylene-2R-octyl-5-oxo-3S-furancarboxylic acid (CAS 191282-48-1) were purchased from Cayman Chemicals.

Techniques: Infection, Cell Culture, Incubation

HEK293T cells were transfected with a Z-Flag MOPV plasmid and HA-NMT1, HA-NMT2 or HA-ITCH plasmids (or control plasmids). Eight hours after transfection, cells were treated (A) or not (B) with AzC12 (10 μM) before mock or IMP1088 treatment (10 nM, 100 nM or 1 μM) for 24h. (A) WCE and IP eluates containing AzC12 incorporated proteins were subjected to “click reaction” with Alk-AF647 for in gel fluorescence detection of protein myristoylation and silver staining for total protein detection. (B) Non AzC12 treated WCE and IP eluates were analyzed by Western Blot as described in . Results shown for A and B are representative of two independent experiments. Lane numbers [ – ] were added to match viewings between gels and panels.

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: HEK293T cells were transfected with a Z-Flag MOPV plasmid and HA-NMT1, HA-NMT2 or HA-ITCH plasmids (or control plasmids). Eight hours after transfection, cells were treated (A) or not (B) with AzC12 (10 μM) before mock or IMP1088 treatment (10 nM, 100 nM or 1 μM) for 24h. (A) WCE and IP eluates containing AzC12 incorporated proteins were subjected to “click reaction” with Alk-AF647 for in gel fluorescence detection of protein myristoylation and silver staining for total protein detection. (B) Non AzC12 treated WCE and IP eluates were analyzed by Western Blot as described in . Results shown for A and B are representative of two independent experiments. Lane numbers [ – ] were added to match viewings between gels and panels.

Techniques: Transfection, Plasmid Preparation, Control, Fluorescence, Silver Staining, Western Blot

(A) A549 cells were infected with MOPV at MOI 0.01 and mock treated or treated with IMP1088, DDD85646 or C75 at 25, 100 or 250 nM. After 48 h of infection, cells were fixed and stained to detect intracellular NP (green channel) and counterstained with DAPI. Results shown are representative of two independent experiments. Scale bar 20 μm. (B) A549 cells were infected with rMOPV Z-Flag or rMOPV mCherryFlag at MOI 0.01 and treated with 10 fold dilutions of IMP1088, DDD85646 or C75 as in . After 48 h of infection, cells were collected, fixed and stained with an anti-Flag-APC conjugated mAb to detect intracellular Z-Flag or mCherryFlag and analyzed by FACS. The results of three independent experiments are represented as mean +/- SEM and expressed as percentage of infected cells. (C) Supernatants from A549 infected with rMOPV Z-Flag (MOI 1) and treated with IMP1088 (50 nM), DDD85646 or C75 (500 nM) were ultracentrifuged (sucrose cushion 20% w/v). Pellets were analyzed by Western Blot for the presence of GP2 or Z-Flag proteins. (D) HEK293T cells were transfected with indicated plasmids and treated or not with IMP1088, DDD85646 or C75 for 24 h. Cell culture supernatants were ultracentrifuged as in (C). WCE and pellets analyzed by Western Blot for the presence of Z-Flag, mCherryFlag and β-actin. Results in C-D are representative of two independent experiments. P <0.001 (***).

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: (A) A549 cells were infected with MOPV at MOI 0.01 and mock treated or treated with IMP1088, DDD85646 or C75 at 25, 100 or 250 nM. After 48 h of infection, cells were fixed and stained to detect intracellular NP (green channel) and counterstained with DAPI. Results shown are representative of two independent experiments. Scale bar 20 μm. (B) A549 cells were infected with rMOPV Z-Flag or rMOPV mCherryFlag at MOI 0.01 and treated with 10 fold dilutions of IMP1088, DDD85646 or C75 as in . After 48 h of infection, cells were collected, fixed and stained with an anti-Flag-APC conjugated mAb to detect intracellular Z-Flag or mCherryFlag and analyzed by FACS. The results of three independent experiments are represented as mean +/- SEM and expressed as percentage of infected cells. (C) Supernatants from A549 infected with rMOPV Z-Flag (MOI 1) and treated with IMP1088 (50 nM), DDD85646 or C75 (500 nM) were ultracentrifuged (sucrose cushion 20% w/v). Pellets were analyzed by Western Blot for the presence of GP2 or Z-Flag proteins. (D) HEK293T cells were transfected with indicated plasmids and treated or not with IMP1088, DDD85646 or C75 for 24 h. Cell culture supernatants were ultracentrifuged as in (C). WCE and pellets analyzed by Western Blot for the presence of Z-Flag, mCherryFlag and β-actin. Results in C-D are representative of two independent experiments. P <0.001 (***).

Techniques: Infection, Staining, Western Blot, Transfection, Cell Culture

(A) A549 cells were infected with rMPOV Z-Flag at MOI 0.01 for 48 h before mock or IMP1088 treatment (50 nM) for the following 48 h. Cells were then stained for the expression of GP2, NP or Z proteins (green) and DAPI. Scale bar 10 μM. (B-C) A549 cells were infected at MOI 0.01 with rMPOV Z-Flag (left panel) or rMOPV NP-HA (right panel) for 48 h before mock or IMP1088 (50 nM), DDD85646 or C75 (500 nM) treatment for 48 h. Permeabilized cells were stained with anti-Flag APC or anti-HA FITC and analyzed by FACS. The results of five (rMPOV Z-Flag) and four (rMPOV NP-HA) independent experiments are expressed in (B) as the mean +/- SEM of the percentage of Fag or HA positive cells and in (C) as the mean of fluorescence intensity (MFI) +/- SEM of Flag or HA positive cells (percentages relative to the mock condition). (D) A549 cells were infected and treated as in (B-C) and WCE were analyzed by Western Blot for the presence of Z-Flag, NP-HA and β-actin. (E) Volcano plot representation of tandem mass spectrometry and comparative proteomics analyses of WCE from A549 cells infected with rMOPV Z-Flag at MOI 1 and treated or not with IMP1088 (50 nM) for 48 h (three independent experiments). (F) Quantification of intracellular mRNA encoding for NP (top) or Z (bottom) from A549 cells infected with rMOPV Z-Flag at MOI 0.01 and mock or IMP1088, DDD85646, C75 treated. mRNAs were quantified at 0 h, 24 h and 48 h post treatment. Results are mean +/- SEM of four independent experiments expressed as mRNA copies standardized to GAPDH levels. P <0.001 (***).

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: (A) A549 cells were infected with rMPOV Z-Flag at MOI 0.01 for 48 h before mock or IMP1088 treatment (50 nM) for the following 48 h. Cells were then stained for the expression of GP2, NP or Z proteins (green) and DAPI. Scale bar 10 μM. (B-C) A549 cells were infected at MOI 0.01 with rMPOV Z-Flag (left panel) or rMOPV NP-HA (right panel) for 48 h before mock or IMP1088 (50 nM), DDD85646 or C75 (500 nM) treatment for 48 h. Permeabilized cells were stained with anti-Flag APC or anti-HA FITC and analyzed by FACS. The results of five (rMPOV Z-Flag) and four (rMPOV NP-HA) independent experiments are expressed in (B) as the mean +/- SEM of the percentage of Fag or HA positive cells and in (C) as the mean of fluorescence intensity (MFI) +/- SEM of Flag or HA positive cells (percentages relative to the mock condition). (D) A549 cells were infected and treated as in (B-C) and WCE were analyzed by Western Blot for the presence of Z-Flag, NP-HA and β-actin. (E) Volcano plot representation of tandem mass spectrometry and comparative proteomics analyses of WCE from A549 cells infected with rMOPV Z-Flag at MOI 1 and treated or not with IMP1088 (50 nM) for 48 h (three independent experiments). (F) Quantification of intracellular mRNA encoding for NP (top) or Z (bottom) from A549 cells infected with rMOPV Z-Flag at MOI 0.01 and mock or IMP1088, DDD85646, C75 treated. mRNAs were quantified at 0 h, 24 h and 48 h post treatment. Results are mean +/- SEM of four independent experiments expressed as mRNA copies standardized to GAPDH levels. P <0.001 (***).

Techniques: Infection, Staining, Expressing, Fluorescence, Western Blot, Mass Spectrometry

(A) Reverse genetics strategy for the expression of Z-Flag and mCherryHA from the MOPV Z ORF and representative immunofluorescence of A549 cells infected with the rMOPV Zflag-P2A-mCherryHA depicting the expression of Z (green) and mCherry (red) and DAPI counterstaining (scale bar 5μm). (B-E) A549 cells were infected with the rMOPV Zflag-P2A-mCherryHA at MOI 2 and Mock treated or treated with IMP1088 (50 nM), DDD85646 or C75 (500 nM). Cells were collected at 5 h, 10 h, 14 h and 24 h post infection for intracellular staining with anti-Flag APC or anti-HA FITC and analyzed by FACS. The results of four independent experiments are in (B) the mean +/- SEM of the percentage of Fag positive cells and in (C) and (D) are the mean of fluorescence intensity (MFI) +/- SEM of Flag or HA positive cells respectively (percentages relative to the mock condition). (E) WCE of experiment set as in (B-D) analyzed by Western Blot for the presence of Z-Flag, mCherryHA and β-actin. Results shown are representative of three independent experiments. P <0.05 (*) and <0.001 (***).

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: (A) Reverse genetics strategy for the expression of Z-Flag and mCherryHA from the MOPV Z ORF and representative immunofluorescence of A549 cells infected with the rMOPV Zflag-P2A-mCherryHA depicting the expression of Z (green) and mCherry (red) and DAPI counterstaining (scale bar 5μm). (B-E) A549 cells were infected with the rMOPV Zflag-P2A-mCherryHA at MOI 2 and Mock treated or treated with IMP1088 (50 nM), DDD85646 or C75 (500 nM). Cells were collected at 5 h, 10 h, 14 h and 24 h post infection for intracellular staining with anti-Flag APC or anti-HA FITC and analyzed by FACS. The results of four independent experiments are in (B) the mean +/- SEM of the percentage of Fag positive cells and in (C) and (D) are the mean of fluorescence intensity (MFI) +/- SEM of Flag or HA positive cells respectively (percentages relative to the mock condition). (E) WCE of experiment set as in (B-D) analyzed by Western Blot for the presence of Z-Flag, mCherryHA and β-actin. Results shown are representative of three independent experiments. P <0.05 (*) and <0.001 (***).

Techniques: Expressing, Immunofluorescence, Infection, Staining, Fluorescence, Western Blot

A549 cells were infected with rMOPV Z-Flag at MOI 2 and subsequently treated or not with IMP1088 (50 nM). MG132, Chloroquine (1 μM or 10 μM) or a mix of both drugs was readily added and incubated for 24 h. WCE were analyzed by Western Blot for the presence of HSP70, LC3I/II, Z-Flag and β-actin. Results are representative of two independent experiments.

Journal: PLOS Pathogens

Article Title: Targeting n-myristoyltransferases promotes a pan- Mammarenavirus inhibition through the degradation of the Z matrix protein

doi: 10.1371/journal.ppat.1012715

Figure Lengend Snippet: A549 cells were infected with rMOPV Z-Flag at MOI 2 and subsequently treated or not with IMP1088 (50 nM). MG132, Chloroquine (1 μM or 10 μM) or a mix of both drugs was readily added and incubated for 24 h. WCE were analyzed by Western Blot for the presence of HSP70, LC3I/II, Z-Flag and β-actin. Results are representative of two independent experiments.

Techniques: Infection, Incubation, Western Blot

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