Journal: Bioactive Materials

Article Title: Energetic metabolism-regulatory glycopeptide hydrogel accelerates pressure ulcer wound repair

doi: 10.1016/j.bioactmat.2026.02.016

Figure Lengend Snippet: GMI gel -mediated metabolic reprogramming and its regulatory role in macrophage polarization. A-D) Hexokinase activity, Phosphofructokinase activity, Isocitrate dehydrogenase activity, and Succinate dehydrogenase activity, n = 3. E-H) Heatmap of LC-MS data and quantitative analysis of the relative abundance of glycolysis and TCA cycle metabolites, n = 3. I) Schematic diagram of glycolysis and the TCA cycle. J) Representative CLSM images of RAW 264.7 cells treated with GMI gel for 72 h. K) Flow cytometry analysis of CD206 and CD86 expression. L, M) The secretion of IL-6 and TNF-α in the culture supernatant of RAW264.7 cells, n = 3. Data are shown as mean ± SDs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, ns, not significant (p > 0.05).

Article Snippet: The expression levels of IL-6 and TNF-α in the culture medium were measured using commercial ELISA kits (Cusabio, Wuhan, China).

Techniques: Activity Assay, Liquid Chromatography with Mass Spectroscopy, Flow Cytometry, Expressing

Journal: Bioactive Materials

Article Title: Energetic metabolism-regulatory glycopeptide hydrogel accelerates pressure ulcer wound repair

doi: 10.1016/j.bioactmat.2026.02.016

Figure Lengend Snippet: GMI gel promotes the healing of infected pressure ulcers in vivo. A) Schematic diagram of GMI gel treatment of infected pressure ulcers. B) Photographs of wounds in mice at different treatment times. C) Signs of wound closure. D) Wound size at different treatment times, n = 3. E) H&E staining images of mouse wound tissue after different treatments on day 12. F) Masson staining images of mouse wound tissue after different treatments on day 12. G) Representative laser Doppler perfusion images of wounds in mice in each treatment group on day 12. H) Representative images of immunohistochemical staining for TNF- α, IL-6 and IL-10 12 days after treatment. I-L) Quantitative statistics of wound site blood perfusion, TNF- α, IL-6 and IL-10, n = 3. Data are shown as mean ± SDs. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The expression levels of IL-6 and TNF-α in the culture medium were measured using commercial ELISA kits (Cusabio, Wuhan, China).

Techniques: Infection, In Vivo, Staining, Immunohistochemical staining

Journal: Food Science & Nutrition

Article Title: Characterization, Identification, and Antioxidant Mechanism of Antioxidant Peptides From Schisandra chinensis Based on Peptidome and Molecular Docking/Dynamics

doi: 10.1002/fsn3.72028

Figure Lengend Snippet: Molecular dynamics simulation analysis between three antioxidant peptides and Keap1. (A–C) RMSD (A), Rg (B) and SASA (C) variations of three complexes over simulation time. (D) RMSF profiles of Keap1 in complexes with FVA, F4, and IL6. (E) Number of hydrogen bonds between Keap1 and three peptides over simulation time.

Article Snippet: IFSPWL (IL6) were synthesized by SynPeptide Co. Ltd. (Nanjing, China).

Techniques:

Journal: Food Science & Nutrition

Article Title: Characterization, Identification, and Antioxidant Mechanism of Antioxidant Peptides From Schisandra chinensis Based on Peptidome and Molecular Docking/Dynamics

doi: 10.1002/fsn3.72028

Figure Lengend Snippet: IL6 antioxidant activity and biological safety. (A, B) The scavenging rates of IL6 on DPPH radicals (A) and ABTS radicals. (C) Hemolytic activity of SCP on mouse red blood cells.

Article Snippet: IFSPWL (IL6) were synthesized by SynPeptide Co. Ltd. (Nanjing, China).

Techniques: Antioxidant Activity Assay, Activity Assay

Journal: Nature Communications

Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer

doi: 10.1038/s41467-026-73931-2

Figure Lengend Snippet: A ELISA detection of IL6 and CCL5 concentrations in the serum of mice bearing A549 High-M tumors. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. B Dual-luciferase reporter assay analyzing the effect of YBX1 overexpression on the activity of the IL6 and CCL5 promoters. Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. C Representative immunofluorescence images of the number of YBX1 condensates in the nuclei of A549 High-M and A549 Low-M cells ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . A549 Low-M : n = 6, minima = 3, maxima = 8, center = 6, Q1/Lower Quartile = 5.25, Q3/Upper Quartile = 6.75, Lower Whisker = 3, Upper Whisker = 8, IQR / Interquartile Range = 1.5; A549 High-M : n = 6, minima = 13, maxima = 26, center = 19.5, Q1/Lower Quartile = 18.25, Q3/Upper Quartile = 23, Lower Whisker = 13, Upper Whisker = 26, IQR / Interquartile Range = 4.75 . D Representative immunofluorescence images of the changes after treatment with 5% 1,6-Hex ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments, Unpaired t test (Two-tailed) . Ctrl: n = 6, minima = 15, maxima = 28, center = 22, Q1/Lower Quartile = 17.5, Q3/Upper Quartile = 25.75, Lower Whisker = 15, Upper Whisker = 28, IQR / Interquartile Range = 28.25; 1,6Hex: n = 6, minima = 2, maxima = 6, center = 3.5, Q1/Lower Quartile = 3, Q3/Upper Quartile = 4.75, Lower Whisker = 2, Upper Whisker = 6, IQR / Interquartile Range = 1.75 . E Representative images of the dynamics of YBX1 condensates in fluorescence bleaching recovery experiment ( Scale bar: 10 μm ). Biological replicates n = 6, independent experiments . F Representative images of nuclear co-localization of YBX1 condensates with transcriptional activity markers p-RNA Pol II (Ser5) and H3K27ac ( Scale bar: 10 μm ). YBX1+Pol II-S5P: n = 20, perspective, minima = 0.825, maxima = 0.987, center = 0.955, Q1/Lower Quartile = 0.90275, Q3/Upper Quartile = 0.97625, Lower Whisker = 0.825, Upper Whisker = 0.987, IQR/Interquartile Range = 0.0735; YBX1 + H3K27ac: n = 20, perspective, minima = 0.814, maxima = 0.985, center = 0.9185, Q1/Lower Quartile = 0.846, Q3/Upper Quartile = 0.95175, Lower Whisker = 0.814, Upper Whisker = 0.985, IQR / Interquartile Range = 0.10575 . G RT-qPCR analysis of IL6 and CCL5 mRNA expression levels in A549 High-M cells treated with 1,6-hexanediol. Biological replicates n = 5, independent experiments, Unpaired t test (Two-tailed); Mean ± SD. H Establish a conditional culture medium experiment: Representative images of TRAP assay analyzing the osteoclast differentiation capacity induced by conditioned medium of A549 cells pre-treated with 1,6-hexanediol ( n = 3, independent experiments), *p < 0.05 ) ( Scale bar: 10 μm ). Biological replicates n = 3, Unpaired t test (Two-tailed); Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator. I Representative images of the activation effect of A549 High-M cell conditioned medium on osteoclasts under IL6 neutralizing antibody (Siltuximab) treatment ( Scale bar: 200 μm ). Biological replicates n = 3, independent experiments, Unpaired t test (Two-tailed) ; Mean ± SD. The schematic diagram was created by the author using Adobe Illustrator.

Article Snippet: For the immunocompetent bone metastasis mouse model, Luci-Lewis lung cancer cells were injected into 4-6-week-old Female C57BL/6 mice (RRID: MGI:7264769) via the tibial metaphysis, with corresponding treatment groups established (e.g., PD-1 mAb, IL6 neutralizing antibody, ICA (MCE, HY-N0678), O-GlcNAcase inhibitor (OGA-i, MCE, HY-12588, etc.).

Techniques: Enzyme-linked Immunosorbent Assay, Two Tailed Test, Luciferase, Reporter Assay, Over Expression, Activity Assay, Immunofluorescence, Whisker Assay, Fluorescence, Quantitative RT-PCR, Expressing, TRAP Assay, Activation Assay

Journal: Nature Communications

Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer

doi: 10.1038/s41467-026-73931-2

Figure Lengend Snippet: A Experimental flowchart: includes tissue collection, multiplex immunofluorescence staining, and automated scanning analysis. The schematic diagram was created by the author using Adobe Illustrator. B Representative multiplex immunofluorescence images showing the infiltration of immune cells in metastatic and non-metastatic tissues ( Scale bar: 2000 μm ). Biological replicates n = 38, patient samples . C Statistical analysis shows the infiltration levels of Treg cells (CD4 + CD25 + FoxP3 + ) and IFN-γ + CD8 + T cells, as well as YBX1 expression levels, in primary tissues from patients without metastasis ( n = 38 ) and with bone metastasis ( n = 38 ). Biological replicates, Unpaired t test (Two-tailed) . For Treg cells: Non-metastasis: n = 38, patient samples, minima = 2.6, maxima = 22.3, center = 11.1, Q1/Lower Quartile = 9.225, Q3/Upper Quartile = 14.45, Lower Whisker = 2.6, Upper Whisker = 22.2875, IQR/Interquartile Range = 5.225; Metastasis: n = 38, patient samples, minima = 4.7, maxima = 29.1, center = 15.85, Q1/Lower Quartile = 10.7, Q3/Upper Quartile = 18.1, Lower Whisker = 4.7, Upper Whisker = 29.1, IQR / Interquartile Range = 7.4; For IFN-γ + CD8 + T cells: Non-metastasis: n = 38, patient samples, minima = 11.8, maxima = 56.5, center = 34, Q1/Lower Quartile = 26.825, Q3/Upper Quartile = 37.575, Lower Whisker = 11.8, Upper Whisker = 53.7, IQR/Interquartile Range = 10.75; Metastasis: n = 38, patient samples, minima = 11.3, maxima = 51.2, center = 21.85, Q1/Lower Quartile = 17.225, Q3/Upper Quartile = 28.975, Lower Whisker = 11.3, Upper Whisker = 46.6, IQR/Interquartile Range = 11.75; For YBX1: Non-metastasis: n = 38,patient samples, minima = 10.8, maxima = 65.2, center = 33.5, Q1/Lower Quartile = 26.95, Q3/Upper Quartile = 39.75, Lower Whisker = 10.8, Upper Whisker = 58.95, IQR/Interquartile Range = 12.8; Metastasis: n = 38, patient samples, minima = 15.3, maxima = 69.2, center = 45.95, Q1/Lower Quartile = 36.15, Q3/Upper Quartile = 53.075, Lower Whisker = 15.3, Upper Whisker = 69.2, IQR/Interquartile Range = 16.925 . The correlation between YBX1 expression and Treg cell infiltration, as well as IFN-γ + CD8⁺ T cell infiltration level. Biological replicates n = 38, patient samples, Pearson’s correlation test (Two-tailed) . D Schematic diagram of mouse model construction and experimental treatments (including sgRNA-mediated YBX1 knockout and immune checkpoint inhibitor therapy). Treatment: IgG/PD-1 mAb 200 µg/mouse (Intraperitoneal injection), ICA 35 mg/kg/day (Intragastric administration). The schematic diagram was created by the author using Adobe Illustrator. E , F Representative flow cytometry plots ( E ) and quantitative analysis ( F ) of the infiltration of IFN-γ + CD8 + T cells and Treg in the tumor microenvironment of bone metastatic lesions. Biological replicates n = 3, mice; One-way ANOVA (multivariate comparison: Tukey) . G Relative levels of IL6 and CCL5 in the serum of mice from different treatment groups. ( n = 5, mice ). Biological replicates n = 5, mice; One-way ANOVA (multivariate comparison: Tukey); Mean ± SD.

Article Snippet: For the immunocompetent bone metastasis mouse model, Luci-Lewis lung cancer cells were injected into 4-6-week-old Female C57BL/6 mice (RRID: MGI:7264769) via the tibial metaphysis, with corresponding treatment groups established (e.g., PD-1 mAb, IL6 neutralizing antibody, ICA (MCE, HY-N0678), O-GlcNAcase inhibitor (OGA-i, MCE, HY-12588, etc.).

Techniques: Multiplex Assay, Immunofluorescence, Staining, Expressing, Two Tailed Test, Whisker Assay, Knock-Out, Injection, Flow Cytometry, Comparison

Journal: Journal of Inflammation Research

Article Title: NHWD-870, a Potent BET Inhibitor, Ameliorated Endotoxemia-Induced Hepatic Inflammation via Suppression of BRD4-STAT1 Signaling and Macrophage M1 Polarization

doi: 10.2147/JIR.S598139

Figure Lengend Snippet: Endotoxemia liver inflammation model was successfully established. ( A) Schematic diagram of LPS modeling (7.5mg/kg). (B) HE staining of liver tissue (magnification 100×). (C and D) Levels of serum ALT( C ) and AST( D ). (E–G) ELISA was used to detect the levels of serum IL6 ( E ), TNFα ( F ), and MCP1 ( G ). (H–J) RT-qPCR was used to detect the mRNA expression of IL6 ( H ), TNFα( I ), and MCP1 ( J ) in the liver tissues of mice. Mice were divided into 3 groups: Saline (n=5), LPS 12h (n=5), LPS 24h (n=5). The data are presented as mean ± SD. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Article Snippet: Mouse IL6 Precoated ELISA Kit (Cat:1210602), and Mouse TNF-α Precoated ELISA Kit (Cat:1217202) were purchased from Dakewe, China, and MCP1 ELISA kits were purchased from RenjieBio, China and operated according to the instructions.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Expressing, Saline

Journal: Journal of Inflammation Research

Article Title: NHWD-870, a Potent BET Inhibitor, Ameliorated Endotoxemia-Induced Hepatic Inflammation via Suppression of BRD4-STAT1 Signaling and Macrophage M1 Polarization

doi: 10.2147/JIR.S598139

Figure Lengend Snippet: NWD-870 improved the survival rate and liver inflammation of mice with endotoxemia. ( A) Schematic diagram of the lethal model of endotoxemia. (B) Survival analysis of mice treated with NHWD-870. Mice were divided into five groups as following: LPS (15mg/kg) +vehicle (n=5), LPS+0.125mg/kg NHWD-870 (n=6), LPS+0.25mg/kg NHWD-870 (n=6), LPS+0.5mg/kg NHWD-870 (n=6), LPS+1.0mg/kg NHWD-870 (n=5). (C) Schematic diagram of NHWD-870 treatment of endotoxemia liver inflammation model. (D and E) Serum levels of ALT and AST. (F) HE staining of liver tissue (Magnification 100×). (G-I) ELISA detection of IL6 ( G ), TNFα ( H ), and MCP1( I ) expression levels in mouse serum. (J-L) RT-qPCR detection of IL6 ( J ), TNFα ( K ), and MCP1 ( L ) mRNA expression levels in mouse liver tissue after NHWD-870 intervention. Each time point of endotoxemia model were divided into 3 groups: vehicle (n=5), NHWD-870 (n=5), JQ1 (n=5). (A and B) showed survival study using 15 mg/kg LPS (lethal dose); (C–L) showed mechanistic study using 7.5 mg/kg LPS (sublethal dose) to enable tissue collection at 12h and 24h. Different doses were selected based on distinct experimental objectives as detailed in Methods. Data are presented as mean ± SD. (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

Article Snippet: Mouse IL6 Precoated ELISA Kit (Cat:1210602), and Mouse TNF-α Precoated ELISA Kit (Cat:1217202) were purchased from Dakewe, China, and MCP1 ELISA kits were purchased from RenjieBio, China and operated according to the instructions.

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

Journal: Journal of Inflammation Research

Article Title: NHWD-870, a Potent BET Inhibitor, Ameliorated Endotoxemia-Induced Hepatic Inflammation via Suppression of BRD4-STAT1 Signaling and Macrophage M1 Polarization

doi: 10.2147/JIR.S598139

Figure Lengend Snippet: Effects of NHWD-870 on inflammatory cell infiltration in endotoxemia mice and its effects on various organs. ( A ) IHC detection of F4/80 expression in liver tissues. (scale bar 100μm) ( B ) Statistics of the positive area of F4/80 IHC staining. ( C ) IHC detection of MPO expression in liver tissues. (scale bar 100μm) ( D ) Statistics of the positive area of MPO IHC staining. IHC positive areas are brown-yellow. Each time point of endotoxemia model were divided into 3 groups: vehicle (n=5), NHWD-870 (n=5), JQ1 (n=5). ( E ) HE staining of NHWD-870 treatment on the heart, liver, spleen, kidney, and liver morphology of mice. (Magnification 100×) (F-G): ALT ( F ) and AST ( G ) levels after NHWD-870 intervention. ( H-J ) ELISA detection of IL6 ( H ), TNFα ( I ), and MCP1 ( J ) levels in mouse serum. Vehicle group (n=5); NHWD-870 group (n=5). Data are presented as mean ± standard deviation. (*p<0.05, ***p<0.001, ****p<0.0001).

Article Snippet: Mouse IL6 Precoated ELISA Kit (Cat:1210602), and Mouse TNF-α Precoated ELISA Kit (Cat:1217202) were purchased from Dakewe, China, and MCP1 ELISA kits were purchased from RenjieBio, China and operated according to the instructions.

Techniques: Expressing, Immunohistochemistry, Staining, Enzyme-linked Immunosorbent Assay, Standard Deviation