Journal: bioRxiv

Article Title: Selenoprotein P Deficiency Drives Hepatocellular Carcinoma Progression via Induction of Neutrophil Senescence and Immunosuppressive Microenvironment

doi: 10.1101/2025.06.24.661430

Figure Lengend Snippet: (a) Flow cytometric analysis of polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) from shNC and sh Sepp1 mice. (b, c) Relative mRNA levels of Cdkn2b (b) and Cdkn2d (c) in neutrophils isolated from shNC and sh Sepp1 animals (n=5-6 per groups). (d) Flow cytometric analysis of T cell proportions in shNC and sh Sepp1 mice. (e-f) CD4 + T cells and CD8 + T cells proportions in the tumor microenvironment of shNC and sh Sepp1 mice. (g) Programmed cell death protein 1 (PD-1) expression in CD8 + T cells, as assessed by flow cytometry. (h) Cytokine staining of intracellular interferon gamma (IFNγ) in CD8 + T cells from shNC and sh Sepp1 mice. (i, j) ELISA quantification of IFNγ (d) and IL-2 (e) in the co-culture spent medium, demonstrating significantly decreased levels of both of these cytokines in cultures employing neutrophils isolated from Sepp1 -depeleted tumors (compared to neutrophils isolated from control tumors) across various neutrophils: OT-1 splenocyte ratios (n = 3 per group). Data are presented as mean ± SEM; statistical significance was determined using two-tailed unpaired Student’s t-tests.

Article Snippet: For assessments of spent medium samples, ELISA was performed using IFNγ (Multi Sciences, Cat. EK280) and IL2 (Multi Sciences, Cat. EK202) ELISA kits according to the manufacturer’s instructions.

Techniques: Derivative Assay, Isolation, Expressing, Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Co-Culture Assay, Control, Two Tailed Test

Journal: Veterinary Sciences

Article Title: Integrated Molecular and Hematobiochemical Biomarkers for the Detection of Bovine Babesiosis in Holstein Calves

doi: 10.3390/vetsci13020176

Figure Lengend Snippet: Differential expression of immune-related genes ( IL-2 , IL-6 , TNF-α , and MCP-1 ) and antioxidant genes ( SOD3 , CAT , GPX , and GST ) in Babesia -affected calves. Data are presented as mean ± SE. An asterisk (*) indicates statistically significant differences ( p < 0.05).

Article Snippet: Cytokines were measured using ELISA kits as follows: IL-2 (RayBiotech, Catalog #: ELB-IL2-1), IL-6 and IL-10 (Boster Biological Technology, Pleasanton, CA, USA.

Techniques: Quantitative Proteomics

Journal: Journal of Inflammation Research

Article Title: Urolithin-C Suppresses Inflammation by Blocking NF-κB Signaling Pathway in LPS-Induced RAW 264.7 Macrophages

doi: 10.2147/JIR.S539273

Figure Lengend Snippet: Urolithin-C’s influence on pro/anti-inflammatory cytokines produced by LPS in RAW 264.7 cells: Following LPS, Positive control (DFC), and Urolithin-C (25 µg/mL) cell culture, ELISA was used to evaluate the production of ( A ) Cox-2, ( B ) IL-2, ( C ) IL-6, ( D ) TNF-α, and ( E ) TGF-β. The mean ± SEM (n = 2) is used to express the results. ** at p ≤ 0.01 and ***at p ≤ 0.001.

Article Snippet: DMEM (Dulbecco’s Modified Eagle Medium) with high glucose medium, Fetal Bovine Serum D-PBS, LPS from E. coli, DMSO (dimethyl sulfoxide), MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide), Antibiotic-Antimycotic solution-100x, Trypsin, EDTA, Tween-20, Diclofenac sodium, Primers-Mouse TNF-alpha, Cox-2, NF-κB, IL6, TGF-beta1 and GAPDH (glyceraldehyde-3-phosphate dehydrogenase), RNAiso plus-Total RNA extraction reagent, TB Green Premix Ex Taq II (Tli RNase H plus), Primer script RT reagent kit (Perfect Real Time), DEPC (Diethyl Pyro carbonate), RayBio Mouse IL-2 ELISA Kit, RayBio Mouse TNF-alpha (Tumor Necrosis Factor alpha) ELISA Kit, RayBio Mouse TGF-beta 1 (Transforming Growth Factor beta-1) ELISA Kit, Mouse IL6 ELISA Kit, Elabscience Mouse PTGS2/Cox-2 ELISA Kit, PE anti NF-κBp65 antibody, Poly-L-Lysine, Fixative solution, DAPI (4’,6-diamidino-2-phenylindole) (hydrochloride), NF-κBp65 (F6) FITC (Fluorescein Isothiocyanate) antibody.

Techniques: Produced, Positive Control, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: iScience

Article Title: Interferon-α and thymosin-α1 plus tislelizumab enhance CD8 + T cell cytotoxicity toward pancreatic ductal adenocarcinoma

doi: 10.1016/j.isci.2025.113053

Figure Lengend Snippet: Influence of tislelizumab, IFN-α, and Tα1 on the surface PD-1 expression of CD8 + T cells (A) Statistical analysis of the number of CD8 + T cells at different times within 14 days after incubation with 1 μg/mL of tislelizumab with or without IFN-α and Tα1 treatment, as determined by a CCK8 assay. (B–D) Statistical analysis of the levels of the secreted cytokines IL-2 (B), TNF-α (C), and IFN-γ (D) in suspensions of cultured CD8 + T cells after incubation with 1 μg/mL of tislelizumab with or without the addition of IFN-α and Tα1 for 14 days, as assayed by ELISA. The data were analyzed by one-way ANOVA and are presented as the means ± SDs of four or five biological replicates. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: IL-2 ELISA Kit , Sino Biological , Cat# KIT11848.

Techniques: Expressing, Incubation, CCK-8 Assay, Cell Culture, Enzyme-linked Immunosorbent Assay