Journal: Bioactive Materials

Article Title: Ameliorating post-infarction myocardial fibrosis and cardiac function via ROS-responsive hydrogel-mediated IL-11 antibody delivery

doi: 10.1016/j.bioactmat.2026.01.041

Figure Lengend Snippet: The expression of interleukin-11 (IL-11) is increased in myocardial fibrosis in vitro and in vivo. A. Western blot analysis of IL-11 and vimentin expression in angiotensin II (AngII, 100 nM) or TGF-β (10 ng/mL)-treated cardiac fibroblasts. B. Quantification of (A). C. TUNEL staining and (D) its quantitative analysis in treated cells. E. CCK-8 viability assays (n = 3). F. Circulating IL-11 levels in mouse blood measured by ELISA (n = 3). ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Following separation by SDS-PAGE and transfer to PVDF membranes, blots were probed overnight with primary antibodies targeting IL-11 (Santa Cruz, sc-133063), Vimentin (Abcam, ab92547), p-ERK1/2 (Sigma-Aldrich, M − 9692), ERK1/2 (Cell Signaling, 4696), and PCNA (Cell Signaling, 2586)

Techniques: Expressing, In Vitro, In Vivo, Western Blot, TUNEL Assay, Staining, CCK-8 Assay, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Ameliorating post-infarction myocardial fibrosis and cardiac function via ROS-responsive hydrogel-mediated IL-11 antibody delivery

doi: 10.1016/j.bioactmat.2026.01.041

Figure Lengend Snippet: Assessment of intrapericardial MAB delivery and P-T@MAB combination therapy in mice. A. Experimental timeline of animal studies. B. In vivo cardiac IVIS imaging following intrapericardial injection of free MAB or P-T@MAB at indicated time points (0 h, 24 h, 2 d, 4 d; n = 3). C. Quantification of cardiac MAB fluorescence. D–I. Serum levels of IL-11, TIMP-1, MMP-2, MMP-9, CCL8, and CXCL2 measured by ELISA (n = 3). n.s., not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Following separation by SDS-PAGE and transfer to PVDF membranes, blots were probed overnight with primary antibodies targeting IL-11 (Santa Cruz, sc-133063), Vimentin (Abcam, ab92547), p-ERK1/2 (Sigma-Aldrich, M − 9692), ERK1/2 (Cell Signaling, 4696), and PCNA (Cell Signaling, 2586)

Techniques: In Vivo, Imaging, Injection, Fluorescence, Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Ameliorating post-infarction myocardial fibrosis and cardiac function via ROS-responsive hydrogel-mediated IL-11 antibody delivery

doi: 10.1016/j.bioactmat.2026.01.041

Figure Lengend Snippet: Western blot and immunofluorescence analysis of myocardial fibrosis markers in myocardial infarction ( MI) mice. A. Western blot analysis for interleukin-11 (IL-11) and vimentin expression in heart tissue of MI mice after treatment with phosphate buffered saline, Gel, MAB, and P-T@MAB and sham group. B-C. Statistical evaluation of IL-11 and vimentin expression. D. Western blot of phosphorylated extracellular signal-regulated kinase (ERK) in heart tissues from MI mice. E.Quantification of (D) (n = 3). F–G. Immunofluorescence staining of α-SMA, collagen Ⅰ, and collagen Ⅲ in scar tissue (H) with corresponding quantitative analysis. Scale bar: 100 μm (n = 5). n.s., not significant; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: Following separation by SDS-PAGE and transfer to PVDF membranes, blots were probed overnight with primary antibodies targeting IL-11 (Santa Cruz, sc-133063), Vimentin (Abcam, ab92547), p-ERK1/2 (Sigma-Aldrich, M − 9692), ERK1/2 (Cell Signaling, 4696), and PCNA (Cell Signaling, 2586)

Techniques: Western Blot, Immunofluorescence, Expressing, Saline, Staining