Journal: Journal of Microbiology and Biotechnology

Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

doi: 10.4014/jmb.2602.02040

Figure Lengend Snippet: ( A ) Western blot detection of PD-1 protein expression; ( B ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( C–E ) IL-6 ( C ), IL-12 ( D ), and TNF-α ( E ) levels measured by ELISA; (F-H) Flow-cytometric detection of IL-6( F ), IL-12( G ), and TNF-α(H) proportions; ( I ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( J ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

Techniques: Western Blot, Expressing, In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Analysis

Journal: Journal of Microbiology and Biotechnology

Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

doi: 10.4014/jmb.2602.02040

Figure Lengend Snippet: ( A ) In vitro bactericidal activity of Kupffer cells against K. pneumoniae evaluated by enumerating viable bacteria; ( B–D ) IL-6 ( B ), IL-12 ( C ), and TNF-α ( D ) levels measured by ELISA; ( E-G ) Flow cytometry detection of IL-6 ( E ), IL-12 ( F ), and TNF-α ( G ) proportions; ( H ) Flow-cytometric analysis of Kupffer-cell phagocytic capacity; ( I ) Western blot detection of SHP2, p-SHP2, STAT1, p-STAT1, NF-κB p65, and p-NF-κB p65. * P < 0.05.

Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

Techniques: In Vitro, Activity Assay, Bacteria, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Analysis, Western Blot

Journal: Journal of Microbiology and Biotechnology

Article Title: Anti-PD-1 Therapy Alleviates Klebsiella pneumoniae -Induced Liver Abscess by Effectively Activating Kupffer Cells

doi: 10.4014/jmb.2602.02040

Figure Lengend Snippet: ( A ) LA severity ameliorated after PD-1 antibody treatment; ( B ) HE staining of mouse liver and microscopic observation; ( C–D ) K. pneumoniae burden in liver ( C ) and blood ( D ) of infected mice. ( E–F ) ELISA detection of mouse serum ALT ( E ) and AST ( F ) levels. * P < 0.05 was considered statistically significant.

Article Snippet: Simultaneously with bacterial inoculation, mice were intraperitoneally injected with 200 μg anti-PD-1 monoclonal antibody (CP151, BioXCell, USA) or an equivalent amount of mouse IgG2a isotype control antibody (BE0085, BioXCell, USA).

Techniques: H&E Stain, Infection, Enzyme-linked Immunosorbent Assay