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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; <t>(D)</t> <t>IFN-γ</t> levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.
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Study group–specific Spearman correlations between liver markers, endotoxemia, and inflammatory <t>markers.</t> <t>IgG</t> represents <t>EndoCAb</t> IgG, and IgM represents EndoCAb IgM
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Study group–specific Spearman correlations between liver markers, endotoxemia, and inflammatory <t>markers.</t> <t>IgG</t> represents <t>EndoCAb</t> IgG, and IgM represents EndoCAb IgM
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Study group–specific Spearman correlations between liver markers, endotoxemia, and inflammatory <t>markers.</t> <t>IgG</t> represents <t>EndoCAb</t> IgG, and IgM represents EndoCAb IgM
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Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; (D) IFN-γ levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.

Journal: Frontiers in Immunology

Article Title: Preliminary evaluation of a DDA cationic liposome-based pulmonary mucosal immunization platform carrying a SARS-CoV-2 spike-derived branched peptide

doi: 10.3389/fimmu.2026.1824741

Figure Lengend Snippet: Expression of co-stimulatory molecules and MHC II on BMDCs after treatment with different formulations. (A) CD86+MHC II+ BMDCs; (B) CD80+MHC II+ BMDCs; (C) CD86+ expression in BMDMs; (D) IFN-γ levels in BMDC culture supernatants; (E) BMDC viability assessed by CCK-8 assay. The treatment groups included PBS, soluble peptide antigen (S450-471), soluble CpG ODN, and S 450-471 @DDA-Lipo+CpG. N = 3 per group. Statistical analysis was performed by one-way ANOVA followed by Dunnett's multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. ns, not significant.

Article Snippet: In addition, IFN-γ levels in the BMDC culture supernatants were measured using a mouse IFN-γ ELISA kit (Lianke Biotech, Hangzhou, China; cat. EK274) according to the manufacturer’s instructions.

Techniques: Expressing, CCK-8 Assay, Control

Systemic humoral immune responses and serum cytokine levels after immunization. (A) Antigen-specific IgG titer in serum; (B) antigen-specific IgG1 titer in serum; (C) antigen-specific IgG2a titer in serum; (D) time-course of serum antigen-specific IgG titer in the IT group only at different immunization time points; (E) serum IFN-γ level; (F) serum granzyme B level; (G) serum IL-4 level; (H) serum IL-10 level. N = 5 per group. Statistical analysis for panels A-C and E-H was performed by one-way ANOVA followed by Dunnett’s multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Frontiers in Immunology

Article Title: Preliminary evaluation of a DDA cationic liposome-based pulmonary mucosal immunization platform carrying a SARS-CoV-2 spike-derived branched peptide

doi: 10.3389/fimmu.2026.1824741

Figure Lengend Snippet: Systemic humoral immune responses and serum cytokine levels after immunization. (A) Antigen-specific IgG titer in serum; (B) antigen-specific IgG1 titer in serum; (C) antigen-specific IgG2a titer in serum; (D) time-course of serum antigen-specific IgG titer in the IT group only at different immunization time points; (E) serum IFN-γ level; (F) serum granzyme B level; (G) serum IL-4 level; (H) serum IL-10 level. N = 5 per group. Statistical analysis for panels A-C and E-H was performed by one-way ANOVA followed by Dunnett’s multiple comparisons test versus the control group. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: In addition, IFN-γ levels in the BMDC culture supernatants were measured using a mouse IFN-γ ELISA kit (Lianke Biotech, Hangzhou, China; cat. EK274) according to the manufacturer’s instructions.

Techniques: Control

Study group–specific Spearman correlations between liver markers, endotoxemia, and inflammatory markers. IgG represents EndoCAb IgG, and IgM represents EndoCAb IgM

Journal: Diabetology & Metabolic Syndrome

Article Title: Continuous glucose monitoring metrics based clustering in people living with type 1 diabetes identifies phenotypes associated with higher inflammation, metabolic dysfunction-associated steatotic liver disease risk, and lower insulin sensitivity

doi: 10.1186/s13098-026-02191-3

Figure Lengend Snippet: Study group–specific Spearman correlations between liver markers, endotoxemia, and inflammatory markers. IgG represents EndoCAb IgG, and IgM represents EndoCAb IgM

Article Snippet: EndoCAb IgG and EndoCAb IgM were measured using Hycult EndoCAb IgG and IgM Elisa kits (HK504-IGG; HK504-IGM; Hycult Biotech, Uden, The Netherlands).

Techniques: