Journal: NPJ Breast Cancer

Article Title: CRISPR screens identify PRMT7 as a therapeutic target to enhance T cell-mediated killing in breast cancer

doi: 10.1038/s41523-025-00888-8

Figure Lengend Snippet: a Inhibition or knockout of the positive control genes, IFNGR2 and JAK2 , and selected sensitizer genes rendered MCF7 cells more resistant to the HER2-restricted T cells (knockout lines shown on top row, inhibition lines along bottom row). b Inhibition or knockout of selected resistor genes rendered MCF7 cells more sensitive to HER2-restricted T cells. Filled circles denote sample means and whiskers represent standard deviations. Significance was computed with two-sample t -tests, using Benjamini–Hochberg correction for multiple testing. All experiments comprised n > 3. (ns = non-significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001).

Article Snippet: After blocking for 1 h in Intercept (TBS) Blocking Buffer (LI-COR Biosciences), membranes were probed overnight at 4 °C with primary antibodies against the respective target: ATF7IP (Sigma-Aldrich, Cat# HPA023505), CFLAR (Cell Signaling Technology, Cat# 56343), CREBBP CBP (D6C5) (Cell Signaling Technology, Cat# 7389), IFNGR2 (OTI1C2) (Origene Technologies Inc, Cat# TA506734S), IRF1 (BioRad Laboratories Inc, Cat# VPA00801 ) and PRMT7 (D1K6R) (Cell Signaling Technology, Cat# 14762).

Techniques: Inhibition, Knock-Out, Positive Control

Journal: Frontiers in Genetics

Article Title: Sex-specific gene expression and weighted co-expression network analysis suggest distinct sex-specific molecular signatures in acutely suicidal MDD-patients without somatic comorbidities

doi: 10.3389/fgene.2025.1653768

Figure Lengend Snippet: Validation of gene expression changes from selected differentially expressed genes in blood of male (A) and female (B) suicidal patients using qRT-PCR. Data are expressed as mean ± SEM. Gene expression changes are expressed as fold change versus the control group (Healthy individuals). Significance was determined with Student’s t-test, with *p < 0.05, **p < 0.01. IL1B = Interleukin 1-beta, IfngR2 = Interferon Gamma Receptor 2, TNFAiP6 = TNF-alpha Induced Protein 6.

Article Snippet: Quantitative PCR was performed using the TaqMan Gene Expression Master Mix (Applied Biosystems) with the following TaqMan gene expression assays: Interleukin 1 beta (Il1b; Hs01555410_m1), Interferon gamma receptor 2 (Ifgnr2; Hs00194264_m1), and TNF alpha-induced protein 6 (Tnfai6; Hs00200180_m1).

Techniques: Biomarker Discovery, Gene Expression, Quantitative RT-PCR, Control

Journal: Journal of Cell Communication and Signaling

Article Title: An immunometabolism‐related signature for renal clear cell carcinoma diagnosis and therapeutic target

doi: 10.1002/ccs3.70047

Figure Lengend Snippet: Immunohistochemical validation of diagnostic model‐related genes. (A) Immunohistochemical staining of APOBEC3G (antibody: HPA001812). (B) Immunohistochemical staining of CD4 (antibody: HPA004472). (C) Immunohistochemical staining of CSK (antibody: HPA026488). (D) Immunohistochemical staining of IFNGR 2 (antibody: HPA001535). (E) Immunohistochemical staining of LDHB (antibody: CAB004641). The left side shows normal kidney tissues ( n = 3) and the right shows KIRC tissues ( n = 3). Staining was performed using DAB (3,3′‐diaminobenzidine) to visualize protein expression levels (brown signals). All images were captured at ×4 magnification. (F) Quantification of IHC staining intensity of APOBEC3G, CD4, CSK, IFNGR2, and LDHB in normal kidney and KIRC tissues ( n = 3 per group). Data are presented as mean ± standard deviation. Statistical analysis was conducted using the t ‐test. ** p < 0.01, *** p < 0.001, **** p < 0.0001. CSK, Cytoskeleton‐Associated Protein; IHC, Immunohistochemistry; KIRC, kidney renal clear cell carcinoma; LDHB, Lactate Dehydrogenase B.

Article Snippet: The membrane was blocked with 5% non‐fat milk in Tris‐Buffered Saline with Tween 20 (TBST) for 1 h, and the membranes were incubated overnight at 4°C with the following primary antibodies: Lactate Dehydrogenase B (LDHB) (1:5000), IFNGR2 (1:1000), CD4 (1:1000), Cytoskeleton‐Associated Protein (CSK) (1:20,000), HLA‐A (1:20,000), APOBEC3G (1:1000, all from Abcam), and the internal control GAPDH (1:2000, Proteintech) at 4°C overnight.

Techniques: Immunohistochemical staining, Biomarker Discovery, Diagnostic Assay, Staining, Expressing, Immunohistochemistry, Standard Deviation

Journal: Journal of Cell Communication and Signaling

Article Title: An immunometabolism‐related signature for renal clear cell carcinoma diagnosis and therapeutic target

doi: 10.1002/ccs3.70047

Figure Lengend Snippet: mRNA and protein expression levels of six key genes in kidney renal clear cell carcinoma cell lines. (A, C, E, G, I, and K) Quantitative real‐time polymerase chain reaction analysis of APOBEC3G, CD4, CSK, HLA‐A, IFNGR2, and LDHB expression, respectively. (B, D, F, H, J, and L) Corresponding western blot analysis of the same six genes. Comparisons were conducted among 786‐O and Caki‐1 renal carcinoma cells and normal human renal tubular epithelial cells (HK‐2). All experiments were performed in triplicate ( n = 3). Data are presented as mean ± standard deviation. Statistical analysis was conducted using the t ‐test. * p < 0.05, ** p < 0.01. CSK, Cytoskeleton‐Associated Protein; LDHB, Lactate Dehydrogenase B.

Article Snippet: The membrane was blocked with 5% non‐fat milk in Tris‐Buffered Saline with Tween 20 (TBST) for 1 h, and the membranes were incubated overnight at 4°C with the following primary antibodies: Lactate Dehydrogenase B (LDHB) (1:5000), IFNGR2 (1:1000), CD4 (1:1000), Cytoskeleton‐Associated Protein (CSK) (1:20,000), HLA‐A (1:20,000), APOBEC3G (1:1000, all from Abcam), and the internal control GAPDH (1:2000, Proteintech) at 4°C overnight.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation