Journal: Frontiers in Physiology

Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

doi: 10.3389/fphys.2026.1906184

Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on pulmonary inflammatory responses. (A) Total cell counts in bronchoalveolar lavage fluid (BALF; n = 8 per group); differential leukocyte counting was not performed. (B–D) Relative mRNA expression levels of IL-4 (B) , IL-5 (C) , and IL-13 (D) in lung tissue (n = 8 per group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; OVA, ovalbumin; SEM, standard error of the mean.

Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

Techniques: Expressing

Journal: Frontiers in Physiology

Article Title: Interleukin-13 R130Q aggravates airway smooth-muscle phenotypic switching and promotes airway remodeling in severe asthma

doi: 10.3389/fphys.2026.1906184

Figure Lengend Snippet: Effects of IL-13 130R and IL-13 130Q on human bronchial smooth muscle cell (hBSMC) phenotypes (n = 4 per group). (A) CCK-8-derived proliferative/metabolic activity. (B) Representative Fluo-3/AM flow-cytometry histograms and median fluorescence intensity quantification of the acetylcholine-induced intracellular calcium response. (C, D) IL-5 (C) and IL-4 (D) concentrations in culture supernatants. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01. IL, interleukin; SEM, standard error of the mean.

Article Snippet: According to the instructions of the manufacturer for the IL-4 and IL-5 ELISA kits (RayBiotech, Peachtree Corners, GA, USA), 100 μL of each sample was added to pre-coated wells and incubated at room temperature for 2.5 h. The wells were then washed three times for 5 min each, and blank wells served as negative controls.

Techniques: CCK-8 Assay, Derivative Assay, Activity Assay, Flow Cytometry, Fluorescence

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: The effect of CoCl 2 on the levels of elabela (ELA), apelin, and apelin receptor APJ. The data were obtained using ELA or apelin ELISA assays in HTR-8/SVneo cell homogenates (Panels A and C) or conditioned media (Panels B and D), western blotting (Panel E), or real-time RT-PCR (Panel F) for APJ. Data are mean ± SEM, n=5-6.

Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Elabela (ELA) or apelin treatment decreased IL-6 mRNA in CoCl 2 -treated HTR-8/SVneo cells. Data are mean ± SEM, n=3-6.

Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

Techniques:

Journal: bioRxiv

Article Title: Complex Modulation of IL-6 Signaling by Apelin and Elabela in HTR-8/SVneo Cells Under Cobalt Chloride-Induced Chemical Hypoxia

doi: 10.64898/2026.08.20.746041

Figure Lengend Snippet: Schematic of proposed interactions between IL-6 signaling and apelin and elabela in HTR-8/SVneo trophoblastic cells under CoCl₂-mimicked hypoxia. We hypothesize that apelin/elabela/APJ axis regulates IL-6 signaling via its actions on various components of the IL-6 classical and trans-signaling pathway.

Article Snippet: Individual ELISA kits were used for IL-6 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6), sIL-6R (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-IL6sR), and sgp130 (RayBiotech Inc., Norcross, GA, USA; cat. No. ELH-sgp130) protein quantification in the conditioned media 24 hours after treatment.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet’s Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential

doi: 10.3390/ijms27156720

Figure Lengend Snippet: Comparison of serum pro-inflammatory cytokine levels between study groups. Box plots for the serum levels (pg/mL) of ( a ) Interleukin-17 (IL-17) and ( b ) Interleukin-6 (IL-6). The horizontal line within each box plot represents the median, and whiskers represent the minimum and maximum values for each cytokine. p < 0.0001: Significant difference versus control group.

Article Snippet: The quantitative measurement of IL-6 and IL-17 concentration was carried out by the ELISA method by using commercial Sandwich ELISA kits (Ray Biotech, Norcross, GA, USA; Cat. No. [ELH-IL-6-CL] and [ELH-IL17]), strictly following the instructions given by the manufacturer.

Techniques: Comparison, Control

Journal: International Journal of Molecular Sciences

Article Title: Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet’s Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential

doi: 10.3390/ijms27156720

Figure Lengend Snippet: ROC curves of serum biomarkers for the diagnosis of BD. The curves represent the sensitivity (y-axis) versus 100%-specificity (x-axis) for each marker. ( a ) MEG3 ( b ) NEAT1 ( c ) miR-124 (AUC = 0.85); ( d ) miR-146a ; ( e ) IL-17; ( f ) IL-6. All biomarkers showed a statistically significant discriminatory power ( p < 0.0001). Abbreviations: AUC, area under the curve.

Article Snippet: The quantitative measurement of IL-6 and IL-17 concentration was carried out by the ELISA method by using commercial Sandwich ELISA kits (Ray Biotech, Norcross, GA, USA; Cat. No. [ELH-IL-6-CL] and [ELH-IL17]), strictly following the instructions given by the manufacturer.

Techniques: Biomarker Discovery, Marker

Journal: International Journal of Molecular Sciences

Article Title: Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet’s Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential

doi: 10.3390/ijms27156720

Figure Lengend Snippet: Machine learning analysis of serum biomarkers. ( a ) Random Forest feature-importance plot showing miR-146a as the most informative individual feature based on the mean decrease in accuracy. ( b ) Decision Tree model identifying serum IL-17 as the root node, indicating that IL-17 provided the most efficient initial split between patients with Behçet’s disease and healthy controls in the final tree model. The Decision Tree was generated using a training set of 76 subjects (63% of the cohort), whereas the remaining 44 subjects were reserved for validation and testing. Consequently, the total number of subjects displayed in the tree diagram is 76 rather than 120.

Article Snippet: The quantitative measurement of IL-6 and IL-17 concentration was carried out by the ELISA method by using commercial Sandwich ELISA kits (Ray Biotech, Norcross, GA, USA; Cat. No. [ELH-IL-6-CL] and [ELH-IL17]), strictly following the instructions given by the manufacturer.

Techniques: Generated, Biomarker Discovery

Journal: International Journal of Molecular Sciences

Article Title: Serum NEAT1/MEG3 and miR-124/miR-146a Dysregulation in Behçet’s Disease: Association with Th17/NF-κB-Related Inflammation and Diagnostic Potential

doi: 10.3390/ijms27156720

Figure Lengend Snippet: Integrated bioinformatic analysis and construction of a putative ncRNA regulatory network associated with Behçet’s disease. ( a , b ) In silico prediction of potential binding sites between lncRNA NEAT1 and miR-146a-5p ( a ) and miR-124-3p ( b ) using the ENCORI database. These analyses identified predicted lncRNA–miRNA binding sites suggesting potential interactions between NEAT1 and the investigated miRNAs. However, these bioinformatic predictions were not experimentally validated in the present study. ( c , d ) Experimentally validated downstream targets retrieved from the miRTarBase database. STAT3 was identified as a validated target of miR-124-3p ( c ), whereas TRAF6 was identified as a validated target of miR-146a-5p ( d ), based on experimentally validated interactions curated in miRTarBase. ( e ) Integrated regulatory network constructed using Cytoscape by combining the experimentally observed expression profiles with bioinformatically predicted lncRNA–miRNA interactions and experimentally validated miRNA–target relationships. The network summarizes the coordinated dysregulation of NEAT1 , MEG3 , miR-124-3p , and miR-146a-5p observed in patients with Behçet’s disease, together with increased circulating IL-17 and IL-6 levels. The connecting edges between NEAT1 and the investigated miRNAs represent bioinformatically predicted interactions retrieved from the ENCORI database and are included to visualize the predicted interaction network. They should not be interpreted as indicating the direction of regulation or as evidence of a direct causal ceRNA mechanism in the current study. In contrast, the interactions between miR-124-3p and STAT3, and between miR-146a-5p and TRAF6, are supported by experimentally validated evidence retrieved from miRTarBase. Collectively, the Cytoscape network integrates our experimental findings with publicly available bioinformatic and experimentally validated data, providing a hypothesis-generating framework for future mechanistic studies rather than a confirmed molecular pathway.

Article Snippet: The quantitative measurement of IL-6 and IL-17 concentration was carried out by the ELISA method by using commercial Sandwich ELISA kits (Ray Biotech, Norcross, GA, USA; Cat. No. [ELH-IL-6-CL] and [ELH-IL17]), strictly following the instructions given by the manufacturer.

Techniques: In Silico, Binding Assay, Construct, Expressing