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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
Basic Fibroblast Growth Factor, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro -derived tanycytes map to the b2 subtype and respond to <t>FGF1</t> (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.
Human Fibroblast Growth Factor, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In vitro -derived tanycytes map to the b2 subtype and respond to FGF1 (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.

Journal: Cell Stem Cell

Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

doi: 10.1016/j.stem.2026.05.005

Figure Lengend Snippet: In vitro -derived tanycytes map to the b2 subtype and respond to FGF1 (A–C) (A) Integration of day 16 data with “tanycytes” cluster from days 25 and 50 + 70 datasets, (B) annotated clusters, and (C) pseudotime trajectory (monocle 3), (1) indicates trajectory root. (D) Gene expression dynamics of selected markers of dotted outline from (C) along pseudotime. (E) Feature plots of key tanycyte markers. (F) Mapping spatial location of the days 50 + 70 tanycyte cluster onto spatial transcriptomic reference of human hypothalamus. (G) ICC on 2D ARC, scale bar: 25 μm. (H) ICC/ISH on xenografts, scale bars: 50 μm. (I) Volcano plot of DEG in tanycytes after 30min stimulation of 50 ng/mL FGF1 in days 50 + 70 cultures ( n = 3 each, RC17). Benjamini-Hochberg corrected p values. (J) DE of EGR1 and FOS across annotated cell types. Significant upregulation in tanycytes shown in red.

Article Snippet: FGF1 (recombinant human) , Miltenyi Biotec , Cat#130-095-789.

Techniques: In Vitro, Derivative Assay, Gene Expression