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Expression of CD68, <t>CD86</t> and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.
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Expression of CD68, <t>CD86</t> and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.
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Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.

Journal: International Journal of Women's Health

Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

doi: 10.2147/IJWH.S567928

Figure Lengend Snippet: Expression of CD68, CD86 and CD206 in decidual tissues of normal pregnancy women and patients with PE. ( A ) The expression levels of CD68, CD86 and CD206 in decidual tissues were assessed by immunohistochemistry (×200). ( B ) Semiquantitative analysis of ( A ). ( C ) The relative protein levels of CD86 and CD206 were detected via Western blotting. GAPDH was used as an endogenous control. ( D ) Semiquantitative analysis of ( C ). ( E ) The relative mRNA levels of TNF-α and IL-6 were detected via q-PCR. Student’s t -test was used for the statistical analysis, and *p<0.05; **p < 0.01.

Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

Techniques: Expressing, Immunohistochemistry, Western Blot, Control

Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.

Journal: International Journal of Women's Health

Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

doi: 10.2147/IJWH.S567928

Figure Lengend Snippet: Immunofluorescence staining for M1 and M2 in normal pregnancy and patients with PE. CD68 is the maker for pan-macrophages. CD80 and CD86 are markers for M1, CD163 and CD206 are markers for M2 macrophages. Scale bars: 100 µm. ( A and B ) The CD68 + CD80 + and CD68 + CD86 + cells in decidual tissues were detected by immunofluorescence double staining. ( C and D ) The CD68 + CD163 + and CD68 + CD206 + cells in decidual tissues were detected by immunofluorescence double staining.

Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

Techniques: Immunofluorescence, Staining, Double Staining

Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.

Journal: International Journal of Women's Health

Article Title: Altered Expression of CXCL16/CXCR6 and Its Correlation with Decidual Macrophage Polarization in Preeclampsia

doi: 10.2147/IJWH.S567928

Figure Lengend Snippet: Decidual macrophages polarization of normal pregnant women and patients with PE. ( A ) Flow cytometric analysis to determine the proportion of M1 (CD68 + CD80 + or CD68 + CD86 + ) and M2 (CD68 + CD163 + or CD68 + CD206 + ) in normal pregnancy and patients with PE. ( B ) Semiquantitative analysis of ( A ). Student’s t -test was used for the statistical analysis, and **p<0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Membranes were blocked for 15 min at room temperature with rapid blocking buffer (NCM Biotech, Suzhou, China), and subsequently incubated with the following primary antibodies: mouse anti-human GAPDH (1:100000, Proteintech, Wuhan, China), rabbit anti-human CD86 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CD206 (1:1000, Proteintech, Wuhan, China), rabbit anti-human CXCL16 (1:1000, Thermo Fisher Scientific, Shanghai, China), and rabbit anti-human CXCR6 (1:1000, Proteintech, Wuhan, China).

Techniques: