Journal: Biochemical and biophysical research communications

Article Title: CD5L is a target of transcription factor Nrf2.

doi: 10.1016/j.bbrc.2025.152225

Figure Lengend Snippet: Fig. 4. Recombinant CD5L (rCD5L) suppresses TGFβ-mediated fibrogenic responses in human lung fibroblast cells. Protein levels of COL1A1, FN1, and α-SMA in IMR-90 cells pre-treated with vehicle or rCD5L (0.4 μg/mL) for 3 h, followed by treatment with vehicle or TGFβ (2 ng/mL) for a further 24 h. Representative blots and quantification relative to α-tubulin are shown. Unpaired two-sample Student’s t-test (2-tailed) was used to analyze the difference between rCD5L-treated and vehicle-treated cells, either without or with TGFβ treatment.

Article Snippet: Human rCD5L (2797-CL, R&D Systems) was reconstituted in sterile PBS.

Techniques: Recombinant

Journal: Autoimmunity

Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

doi: 10.1080/08916934.2023.2201412

Figure Lengend Snippet: Figure 2. CD5L induced the expression of inflammatory factors in RA-FLS. The cells were stimulated with different concentrations of recombinant CD5L (0, 50, 100, 200, 500, 1000 ng/mL) for 24 h. (A) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. (C) The levels of IL-6, IL-8, and TNF-α in cell culture supernatant after stimulating different time. (D) mRNA levels of IL-6, IL-8, and TNF-α after stimulating different time. The concentration of CD5L for stimulation was 500 ng/mL. Compared with the control group (0 ng/mL), ns, no statistical difference; *p < 0.05, **p < 0.01, ***p < 0.001. The measurements were repeated three times for each group of data.

Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

Techniques: Expressing, Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Concentration Assay, Control

Journal: Autoimmunity

Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

doi: 10.1080/08916934.2023.2201412

Figure Lengend Snippet: Figure 3. CD5L activated ERK1/2 MAPK signaling pathway. Cells (5 × 106) were challenged with CD5L protein (500 ng/mL) for different times(0, 30, 60, 90 min). The protein was extracted and the expression levels of phosphorylation signal molecules (A) p-p38 MAPK, p-AKT, p-JAK, and p-I κB-α and (B) p-ERK were detected by WB.

Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

Techniques: Expressing, Phospho-proteomics

Journal: Autoimmunity

Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

doi: 10.1080/08916934.2023.2201412

Figure Lengend Snippet: Figure 4. Effect of inhibitor on CD5L-induced inflammatory-related factors in RA-FLS. The cells were pretreated with inhibitor U0126 for 1 h and cultured for 24 h with or without CD5L. The optimum concentration of U0126 deter mined by pre-experiment is 16 μM. (A) The expression levels of IL-6, IL-8, and TNF-α in the supernatant of cell culture were detected by ELISA. (B) mRNA levels of IL-6, IL-8, and TNF-α were detected by RT-PCR. Results of three independent replicates were expressed as mean ± standard deviation. Figure 4. Continued.

Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

Techniques: Cell Culture, Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Standard Deviation

Journal: Autoimmunity

Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

doi: 10.1080/08916934.2023.2201412

Figure Lengend Snippet: Figure 6. Effects of CD5L on apoptosis and proliferation of RA-FLS. Cells with inhibitor U0126 were pretreated for 1 h and cultured for 24 h with or without CD5L. (A) mRNA levels of BAX and BCL2 were detected by RT-PCR; (B) BAX and BCL-2 protein levels were detected by WB. (C) CCK-8 kit was used to detect the proliferation ability of RA-FLS. The blank wells contained only culture medium, and the control wells contained cells and culture medium. The exper imental results were expressed as mean ± standard deviation.

Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, CCK-8 Assay, Control, Standard Deviation

Journal: Autoimmunity

Article Title: CD5L induces inflammation and survival in RA-FLS through ERK1/2 MAPK pathway.

doi: 10.1080/08916934.2023.2201412

Figure Lengend Snippet: Figure 5. Effect of inhibitor on CD5L activation of ERK1/2 MAPK signaling pathway. Cells (5 × 106) were treated with ERK1/2 inhibitor U0126 for 1 h, and then added CD5L protein for 90 min. Protein was extracted, and the expression level of phosphorylated signal molecule p-ERK1/2 MAPK was detected by WB. GAPDH was used to correct the protein content of each sample.

Article Snippet: Materials Recombinant human CD5L protein was purchased from R&D Systems (Minneapolis, USA).

Techniques: Activation Assay, Expressing

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: The Up-Regulated and Down-Regulated Top 5 Proteins Between the Two Groups

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Clinical Proteomics

Journal: Journal of Inflammation Research

Article Title: Serum Proteomic Analysis Revealed Biomarkers for Eosinophilic Chronic Rhinosinusitis with Nasal Polyps Pathophysiology

doi: 10.2147/JIR.S444280

Figure Lengend Snippet: Validation of the top 5 up and down-regulated proteins in an independent validation cohort. ( A – E ) comparison of serum MRC1, CDH13, LTA4H, CD5L and MMP2 concentrations between the eCRSwNP and neCRSwNP groups. ( F – J ) comparison of serum SERPING1, IGFBP5, TRIM28, CHL1 and FABP5 levels between the eCRSwNP and neCRSwNP groups. *P<0.05; **P<0.01; ***P<0.001.

Article Snippet: Mannose receptor C-type 1 (MRC1) kits (Cat: CSB-E09961h), cadherin 13 (CDH13) kits (Cat: CSB-E13817h), cluster of differentiation 5 antigen-like (CD5L) kits (Cat: CSB-E13423h), matrix metalloproteinase-2 (MMP2) kits (Cat: CSB-E04675h), plasma protease C1 inhibitor (SERPING1) kits (Cat: CSB-EL021086HU), insulin-like growth factor-binding protein 5 (IGFBP5) kits (Cat: CSB-EL010901HU), tripartite motif-containing protein 28 (TRIM28) kits (Cat: CSB-EL024502HU), and fatty acid-binding protein 5 (FABP5) kits (Cat: CSB-EL007946HU) were purchased from Cusabio (Wuhan, China).

Techniques: Biomarker Discovery, Comparison