Journal: Biology Direct

Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination

doi: 10.1186/s13062-025-00677-0

Figure Lengend Snippet: ( A ) Cell viability of SKMES1 cells treated with ICG-001. ( B ) RNA level of CCL2 in SKMES1 cells. ( C ) Protein level of CCL2 in SKMES1 cells. ( D ) Cellular migration and invasion ability of THP-1 cells analyzed by transwell assay. ( E ) RNA levels of Arg-1, CD163 and IL-10 in M0 THP-1 cells treated with SKMES1 CM. ( F ) The proportion of CD206 + cells in M0 THP-1 cells with SKMES1 CM treatment. EV: empty vector, SR oe : SH3RF3 overexpression. **: p < 0.01, ***: p < 0.001

Article Snippet: Colonies were fixed with 4% paraformaldehyde at room temperature for 25 min and stained with crystal violet dye for 5 min. CCL2 levels were determined using the Human CCL2 ELISA Kit (Lianke Bio, China).

Techniques: Migration, Transwell Assay, Plasmid Preparation, Over Expression

Journal: Biology Direct

Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination

doi: 10.1186/s13062-025-00677-0

Figure Lengend Snippet: ( A & B ) SKMES1 cells with SH3RF2 overexpression or knockdown were injected subcutaneously into the right armpit of Balb/c nude mice. Tumor size was measured every 3 days. Tumors were removed and weighed after 33 days. ( C & D ) The protein level of CCL2 in tumor determined by ELISA. ( E ) Detection of SH3RF2 expression in tumor tissues by immunohistochemistry, bar = 50 μm. ( F ) Immunohistochemistry for Ki-67 in tumor tissues, bar = 50 μm. ( G ) The proportion of CD11b + F4/80 + CD206 + cells in tumor. NC sh : negative control shRNA, SR sh−1 : SH3RF2 shRNA-1, SR sh−2 : SH3RF2 shRNA-2, EV: empty vector, SR oe : SH3RF3 overexpression. **: p < 0.01, ***: p < 0.001

Article Snippet: Colonies were fixed with 4% paraformaldehyde at room temperature for 25 min and stained with crystal violet dye for 5 min. CCL2 levels were determined using the Human CCL2 ELISA Kit (Lianke Bio, China).

Techniques: Over Expression, Knockdown, Injection, Enzyme-linked Immunosorbent Assay, Expressing, Immunohistochemistry, Negative Control, shRNA, Plasmid Preparation

Journal: Biology Direct

Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination

doi: 10.1186/s13062-025-00677-0

Figure Lengend Snippet: ( A ) Protein level of LZTS2 in SKMES1 cells with SH3RF2 and LZTS2 overexpression. ( B ) Cell viability of SKMES1 cells with SH3RF2 and LZTS2 overexpression. ( C ) Immunofluorescence detection of the distribution of β-catenin in SKMES1 cells and quantitative analysis of nuclear-to-cytoplasmic ratio of β-catenin fluorescence intensity. ( D ) Protein level of CCL2 in SKMES1 cells. ( E ) RNA levels of Arg-1, CD163 and IL-10 in M0 THP-1 cells with SKMES1 CM treatment. ( F ) Cellular migration and invasion ability of M0 THP-1 cells with SKMES1 CM treatment. ( G ) The proportion of CD206 + cells in the M0 THP-1 cells treated with SKMES1 CM. EV: empty vector, SR oe : SH3RF3 overexpression. LZ oe : LZTS2 overexpression. **: p < 0.01, ***: p < 0.001

Article Snippet: Colonies were fixed with 4% paraformaldehyde at room temperature for 25 min and stained with crystal violet dye for 5 min. CCL2 levels were determined using the Human CCL2 ELISA Kit (Lianke Bio, China).

Techniques: Over Expression, Immunofluorescence, Fluorescence, Migration, Plasmid Preparation