Review





Similar Products

horizontal puller  (Sutter Instrument Company)
96
Sutter Instrument Company horizontal puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Horizontal Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-1000+Next+Generation+Micropipette+Puller/pmc13635928-80-13-16
Average 96 stars, based on 1 article reviews
horizontal puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
sutter p 1000 programmable horizontal micropipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company sutter p 1000 programmable horizontal micropipette puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sutter P 1000 Programmable Horizontal Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-1000+Next+Generation+Micropipette+Puller/pm42768120-268-26-32
Average 96 stars, based on 1 article reviews
sutter p 1000 programmable horizontal micropipette puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
horizontal pipette puller p 97  (Sutter Instrument Company)
96
Sutter Instrument Company horizontal pipette puller p 97
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Horizontal Pipette Puller P 97, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pmc13616201-43-8-12
Average 96 stars, based on 1 article reviews
horizontal pipette puller p 97 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
horizontal pipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company horizontal pipette puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Horizontal Pipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pmc13628827-119-6-10
Average 96 stars, based on 1 article reviews
horizontal pipette puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
flaming brown horizontal puller  (Sutter Instrument Company)
96
Sutter Instrument Company flaming brown horizontal puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Flaming Brown Horizontal Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pm41196298-54-18-22
Average 96 stars, based on 1 article reviews
flaming brown horizontal puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
sutter p 1000 horizontal micropipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company sutter p 1000 horizontal micropipette puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Sutter P 1000 Horizontal Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-1000+Next+Generation+Micropipette+Puller/pmc13621867-34-28-33
Average 96 stars, based on 1 article reviews
sutter p 1000 horizontal micropipette puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
horizontal p 97 flaming brown micropipette puller  (Sutter Instrument Company)
96
Sutter Instrument Company horizontal p 97 flaming brown micropipette puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Horizontal P 97 Flaming Brown Micropipette Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pm41147441-201-10-17
Average 96 stars, based on 1 article reviews
horizontal p 97 flaming brown micropipette puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
flaming brown horizontal electrode puller  (Sutter Instrument Company)
96
Sutter Instrument Company flaming brown horizontal electrode puller
mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. <t>Horizontal</t> bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Flaming Brown Horizontal Electrode Puller, supplied by Sutter Instrument Company, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/horizontal+micropipette+puller/P-97+Flaming%2FBrown+Type+Micropipette+Puller/pmc12440207-236-6-12
Average 96 stars, based on 1 article reviews
flaming brown horizontal electrode puller - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier

Image Search Results


mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. Horizontal bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Neurobiology of disease

Article Title: Selective impairment of long-term depression in accumbal D1R+ MSNs involves calcium-permeable AMPARs in early Alzheimer's disease

doi: 10.1016/j.nbd.2026.107568

Figure Lengend Snippet: mGluR-dependent LTD is selectively impaired in D1R+ MSNs, and residual synaptic transmission is selectively NASPM-sensitive in the NAc core of 6-month-old APP/PS1 mice. (A) Schematic representation of whole-cell voltage-clamp recordings of AMPAR-mediated eEPSCs in NAc core MSNs from 6-month-old WT;D1-tdTomato and APP/PS1;D1-tdTomato mice. Recordings were performed using a CsCl-based internal solution containing QX-314 and TEA, in ACSF containing picrotoxin and D-APV. After a 10-min baseline period, mGluR-dependent LTD was induced by bath application of DHPG for 5 min. NASPM was subsequently applied from 35 to 50 min. Period 1 corresponds to baseline (0–10 min), period 2 to the final 5 min before NASPM application (30–35 min), used to quantify DHPG-induced LTD, and period 3 to the final 5 min of NASPM application (45–50 min). (B) Representative paired-pulse AMPAR-mediated eEPSCs recorded from D1R+ and D1R− MSNs in WT;D1-tdTomato (black) and APP/PS1;D1-tdTomato (red) mice during baseline (1), after DHPG-induced LTD (2), and during NASPM application (3). Scale bars: 50 pA, 40 ms. (C) Time course of normalized eEPSC amplitude in D1R+ and D1R− MSNs. DHPG-induced LTD was impaired in APP/PS1 D1R+ MSNs, whereas D1R− MSNs showed comparable synaptic depression between genotypes. Subsequent NASPM application produced an additional reduction in residual eEPSC amplitude selectively in APP/PS1 D1R+ MSNs. Horizontal bars indicate the periods of DHPG and NASPM application. Symbols and shaded regions represent mean ± SD. (D) Quantification of normalized eEPSC amplitude during baseline (1; 0–10 min), after DHPG-induced LTD (2; 30–35 min), and during the final 5 min of NASPM application (3; 45–50 min). The mixed-effects model showed significant effects of experimental stage and group, as well as a significant stage × group interaction (stage, F(1.841,38.67) = 137.8, p < 0.001; experimental group, F(3,21) = 4.459, p = 0.014; stage × experimental group interaction, F(5.524,38.67) = 5.484, p < 0.001). Tukey's multiple-comparisons test showed significant LTD relative to baseline in WT D1R+ (p < 0.001), WT D1R− (p < 0.001), and APP/PS1 D1R− MSNs (p < 0.001), whereas no significant depression was detected in APP/PS1 D1R+ MSNs after DHPG application ( p = 0.293). During the LTD period, normalized eEPSC amplitude was higher in APP/PS1 D1R+ MSNs than in WT D1R+ MSNs ( p = 0.031) and APP/PS1 D1R− MSNs (p = 0.014), whereas WT and APP/PS1 D1R− MSNs did not differ ( p = 0.995). NASPM significantly reduced residual eEPSC amplitude in APP/PS1 D1R+ MSNs relative to the LTD period ( p = 0.020) but produced no additional significant inhibition in WT D1R+ ( p = 0.939), WT D1R− ( p = 0.788), or APP/PS1 D1R− MSNs ( p = 0.921). No significant differences among groups remained during the final NASPM period. (E) Paired-pulse ratio expressed as the ratio between post-LTD and pre-LTD values. No significant differences were detected among groups (Kruskal–Wallis test: H(3) = 2.02, p = 0.570). Each point represents one neuron recorded from a separate acute brain slice, with only one neuron recorded per slice. For panels C–D, the numbers of neurons/mice were: WT D1R+, n = 7/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. For panel E, the numbers of neurons/mice were: WT D1R+, n = 6/4; WT D1R−, n = 5/4; APP/PS1 D1R+, n = 7/5; and APP/PS1 D1R−, n = 6/5. Bars and error bars represent mean ± SD, and individual data points are shown. * p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Recording pipettes (4–5 MΩ) were pulled from borosilicate glass capillaries (WPI) using a horizontal puller (P-1000, Sutter Instruments).

Techniques: Transmission Assay, Transcranial Magnetic Stimulation, Produced, Inhibition, Slice Preparation