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Journal: iScience
Article Title: NEK2A regulates PLIN2 expression through a SERBP1 dependent pathway
doi: 10.1016/j.isci.2026.116254
Figure Lengend Snippet: The effects of SERBP1 depletion on PPARγ target genes in HepG2 cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
Article Snippet: HuH7 cells were obtained from JCRB Cell Bank, and
Techniques: shRNA, Incubation, Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing