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ATCC hepg2 cells
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
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CLS Cell Lines Service GmbH hepg2 cells
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
Hepg2 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hep g2  (ATCC)
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ATCC hep g2
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
Hep G2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Procell Inc hepg2 human hepatocellular carcinoma cells
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
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ATCC human hepg2 hepatocarcinoma cells
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
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ATCC human hcc cell lines hepg2
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
Human Hcc Cell Lines Hepg2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Procell Inc hepatocellular carcinoma cell lines hepg2
The effects of SERBP1 depletion on PPARγ target genes in <t>HepG2</t> cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.
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The effects of SERBP1 depletion on PPARγ target genes in HepG2 cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.

Journal: iScience

Article Title: NEK2A regulates PLIN2 expression through a SERBP1 dependent pathway

doi: 10.1016/j.isci.2026.116254

Figure Lengend Snippet: The effects of SERBP1 depletion on PPARγ target genes in HepG2 cells HepG2 cells were treated with scrambled short hairpin RNA (sh Scr) or shRNA against SERBP1 (sh SERBP ). The cells were incubated with or without 0.5 mM oleic acid (OA). Total RNA was isolated, and RT-PCR was performed to determine the mRNA levels of SERBP1 (A), PLIN2 (B), PLIN3 (C), and CD36 (D), normalized to GAPDH . The expression level in OA-treated sh Scr was set to 1.0. The results are shown as scattered dot plots (mean ± SD). ∗∗ p < 0.01, one-way ANOVA followed by Tukey-Kramer post hoc test.

Article Snippet: HuH7 cells were obtained from JCRB Cell Bank, and HepG2 cells were obtained from ATCC.

Techniques: shRNA, Incubation, Isolation, Reverse Transcription Polymerase Chain Reaction, Expressing