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Selection of <t>T1D</t> TCR that mimic MS TCR. Note that many of the MS TCR are identified as being MBP specific. A complete listing of all the T1D–MS TCR matches found is supplied in the . The titles of the TCR (e.g., T1D DIA 11) refer to the identities of the TCR sequences provided in and are followed by the TCR sequence. The underlined codes are the UNIPROT KB identification numbers of the MS TCR sequences.
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Selection of T1D TCR that mimic MS TCR. Note that many of the MS TCR are identified as being MBP specific. A complete listing of all the T1D–MS TCR matches found is supplied in the . The titles of the TCR (e.g., T1D DIA 11) refer to the identities of the TCR sequences provided in and are followed by the TCR sequence. The underlined codes are the UNIPROT KB identification numbers of the MS TCR sequences.

Journal: Cells

Article Title: Type 1 Diabetes-Associated Autoantibodies, Sera and T-Cell Receptor Sequences Recognize Myelin Basic Protein: A Novel Peripheral Diabetic Neuropathy–Multiple Sclerosis Connection

doi: 10.3390/cells15151384

Figure Lengend Snippet: Selection of T1D TCR that mimic MS TCR. Note that many of the MS TCR are identified as being MBP specific. A complete listing of all the T1D–MS TCR matches found is supplied in the . The titles of the TCR (e.g., T1D DIA 11) refer to the identities of the TCR sequences provided in and are followed by the TCR sequence. The underlined codes are the UNIPROT KB identification numbers of the MS TCR sequences.

Article Snippet: Disease State Supplier Our ID Sample ID Age Sex Race HbA1c % Serum Treatment T1D Lee Biosolutions LEE 01 09E5731 A1c-50.11 39 M Caucasian 11.5 EDTA T1D Lee Biosolutions LEE 02 09E5731 A1c-50.12 36 F Caucasian 14.4 EDTA T1D Lee Biosolutions LEE 03 09E5731 A1c-03 29 F Caucasian 10.3 EDTA T1D Lee Biosolutions LEE 04 09E5731 A1c-04 30 M Caucasian 10.5 EDTA T1D Lee Biosolutions LEE 05 09E5731 A1c-05 24 F Caucasian 11.3 EDTA T1D Innovative Research #69 HMN889069 61 M Caucasian Off the clot T1D Innovative Research #70 HMN889070 36 M Asian Off the clot T1D Innovative Research #71 HMN889071 56 F Caucasian Off the clot Healthy Zen-Bio Healthy 1 HSER-2ML ?

Techniques: Selection, Sequencing

Ultraviolet spectroscopy experiment evaluating the binding of type 1 diabetic T cell receptor sequences (DIA TCR) to human myelin basic protein (hMBP). Binding constants can be approximated by finding the concentration of TCR peptide at the inflection point of each curve. However, because these experiments were run in solution, while actual TCR-MBP binding would involve cell-cell interactions, the calculated affinities probably significantly underestimate the actual affinity of the TCR for MBP in vivo. The TCR labels refer to the sequences listed in and . TCR4,8,9 is a consensus sequence derived from DIA4, DIA8, and DIA9.

Journal: Cells

Article Title: Type 1 Diabetes-Associated Autoantibodies, Sera and T-Cell Receptor Sequences Recognize Myelin Basic Protein: A Novel Peripheral Diabetic Neuropathy–Multiple Sclerosis Connection

doi: 10.3390/cells15151384

Figure Lengend Snippet: Ultraviolet spectroscopy experiment evaluating the binding of type 1 diabetic T cell receptor sequences (DIA TCR) to human myelin basic protein (hMBP). Binding constants can be approximated by finding the concentration of TCR peptide at the inflection point of each curve. However, because these experiments were run in solution, while actual TCR-MBP binding would involve cell-cell interactions, the calculated affinities probably significantly underestimate the actual affinity of the TCR for MBP in vivo. The TCR labels refer to the sequences listed in and . TCR4,8,9 is a consensus sequence derived from DIA4, DIA8, and DIA9.

Article Snippet: Disease State Supplier Our ID Sample ID Age Sex Race HbA1c % Serum Treatment T1D Lee Biosolutions LEE 01 09E5731 A1c-50.11 39 M Caucasian 11.5 EDTA T1D Lee Biosolutions LEE 02 09E5731 A1c-50.12 36 F Caucasian 14.4 EDTA T1D Lee Biosolutions LEE 03 09E5731 A1c-03 29 F Caucasian 10.3 EDTA T1D Lee Biosolutions LEE 04 09E5731 A1c-04 30 M Caucasian 10.5 EDTA T1D Lee Biosolutions LEE 05 09E5731 A1c-05 24 F Caucasian 11.3 EDTA T1D Innovative Research #69 HMN889069 61 M Caucasian Off the clot T1D Innovative Research #70 HMN889070 36 M Asian Off the clot T1D Innovative Research #71 HMN889071 56 F Caucasian Off the clot Healthy Zen-Bio Healthy 1 HSER-2ML ?

Techniques: Spectroscopy, Binding Assay, Concentration Assay, In Vivo, Sequencing, Derivative Assay

Results of enzyme-linked immunoadsorption assays involving sera from type 1 diabetes mellitus patients (T1D) or healthy controls binding to human myelin basic protein (hMBP). DIA 1, DIA 2, etc. refer to the sera codes (see Materials and Methods). Only some of the experimental curves are provided so that the differences in the curves can be seen clearly. In particular, most of the negative results (flat lines) were omitted because they overlay each other to such an extent that they cannot be differentiated. This omitted data and the resulting binding constants are shown in .

Journal: Cells

Article Title: Type 1 Diabetes-Associated Autoantibodies, Sera and T-Cell Receptor Sequences Recognize Myelin Basic Protein: A Novel Peripheral Diabetic Neuropathy–Multiple Sclerosis Connection

doi: 10.3390/cells15151384

Figure Lengend Snippet: Results of enzyme-linked immunoadsorption assays involving sera from type 1 diabetes mellitus patients (T1D) or healthy controls binding to human myelin basic protein (hMBP). DIA 1, DIA 2, etc. refer to the sera codes (see Materials and Methods). Only some of the experimental curves are provided so that the differences in the curves can be seen clearly. In particular, most of the negative results (flat lines) were omitted because they overlay each other to such an extent that they cannot be differentiated. This omitted data and the resulting binding constants are shown in .

Article Snippet: Disease State Supplier Our ID Sample ID Age Sex Race HbA1c % Serum Treatment T1D Lee Biosolutions LEE 01 09E5731 A1c-50.11 39 M Caucasian 11.5 EDTA T1D Lee Biosolutions LEE 02 09E5731 A1c-50.12 36 F Caucasian 14.4 EDTA T1D Lee Biosolutions LEE 03 09E5731 A1c-03 29 F Caucasian 10.3 EDTA T1D Lee Biosolutions LEE 04 09E5731 A1c-04 30 M Caucasian 10.5 EDTA T1D Lee Biosolutions LEE 05 09E5731 A1c-05 24 F Caucasian 11.3 EDTA T1D Innovative Research #69 HMN889069 61 M Caucasian Off the clot T1D Innovative Research #70 HMN889070 36 M Asian Off the clot T1D Innovative Research #71 HMN889071 56 F Caucasian Off the clot Healthy Zen-Bio Healthy 1 HSER-2ML ?

Techniques: Binding Assay