Journal: Biomedical Optics Express

Article Title: Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules

doi: 10.1364/BOE.596597

Figure Lengend Snippet: Fabrication process of HCF- and MMF-SERS sensors. The length of two SERS sensors was designed as 1 cm. GNPs were found to be randomly distributed on the inner walls for HCF sample and on the fiber tip surface for MMF sample, respectively.

Article Snippet: HCF (HC-1550-02) and MMF (FG105LCA) were purchased from Thorlabs, USA.

Techniques:

Journal: Biomedical Optics Express

Article Title: Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules

doi: 10.1364/BOE.596597

Figure Lengend Snippet: FE-SEM images of the SERS sensors fabricated with HCF and MMF. Using the CSA technique, GNPs were found to be randomly distributed on the inner walls for HCF sample and on the fiber tip surface for MMF sample, respectively.

Article Snippet: HCF (HC-1550-02) and MMF (FG105LCA) were purchased from Thorlabs, USA.

Techniques:

Journal: Biomedical Optics Express

Article Title: Synergistic combination of convective self-assembly and hollow core fiber for sensitive SERS detection of glucose molecules

doi: 10.1364/BOE.596597

Figure Lengend Snippet: (a) Transmittance test results for HCF and MMF samples when the light source with a power of 7.7 mW was coupled to the 3-cm long fibers. The measured transmission power was 3.51 ± 0.024 mW for HCF and 1.90 ± 0.072 mW for MMF. (b) The forbidden zone and allowed zone of the commercial HCF sample. When the hollow core is filled with air, the resonance frequencies range from 1490 to 1680 nm. (c) When the hollow core is filled with glucose solution, this leads to a shift of the resonance wavelengths, causing a redistribution of the allowed transmission band. The allowed zone has a range from 820 to 924 nm and this band covers the wavelengths of Raman signal.

Article Snippet: HCF (HC-1550-02) and MMF (FG105LCA) were purchased from Thorlabs, USA.

Techniques: Transmission Assay

Journal: International Journal of Molecular Sciences

Article Title: Atorvastatin Attenuates Human Cardiac Fibroblast Activation, with Associated Changes in GATA4/MEF2C and Selected Fibrosis-Related microRNAs

doi: 10.3390/ijms27094146

Figure Lengend Snippet: Viability of HCFs after treatment with atorvastatin, assessed by flow cytometry and MTT assay. ( A ). Flow cytometric analysis using PI staining showed that the proportion of viable (PI-negative) HCFs remained above 90% after 24, 48, and 72 h of atorvastatin (ATV) treatment ( n = 3). ( B ). MTT assay confirmed sustained metabolic activity of HCFs under the same conditions, with cell viability consistently exceeding 90% at all time points ( n = 8). Data are presented as mean ± SD. ATV = atorvastatin; CTRL = control.

Article Snippet: The expression of surface antigens (CD105 + , CD90 + , CD73 + , CD14 − , CD20 − , CD34 − , CD45 − ) in the HCFs was analysed by flow cytometry using a MACSQuant Analyzer 10 flow cytometer (Cat# 130-096-343, Miltenyi Biotec, Bergisch Gladbach, Germany), MACSQuantify software version 2.13.3 (Miltenyi Biotec) and MSC Phenotyping Kit (Cat# 130-095-198, Miltenyi Biotec), following the manufacturer’s instructions.

Techniques: Flow Cytometry, MTT Assay, Staining, Activity Assay, Control

Journal: International Journal of Molecular Sciences

Article Title: Atorvastatin Attenuates Human Cardiac Fibroblast Activation, with Associated Changes in GATA4/MEF2C and Selected Fibrosis-Related microRNAs

doi: 10.3390/ijms27094146

Figure Lengend Snippet: Mesenchymal identity of HCFs following atorvastatin treatment. ( A ). Representative flow cytometry histograms showing expression of surface markers after 48 h of atorvastatin exposure. Blue/dark histograms represent the isotype control, while orange/red histograms represent the atorvastatin-treated HCFs. HCFs remained positive for CD73 and CD105 and partially expressed CD90. Hematopoietic and endothelial markers (CD14, CD20, CD34, CD45) were consistently negative. ( B ). Quantitative summary of surface marker expression showing the average percentage of HCFs positive for mesenchymal markers (CD73, CD105, CD90) and negative for hematopoietic/endothelial markers. Data are presented as mean ± SD ( n = 3). ATV = atorvastatin; CTRL = control.

Article Snippet: The expression of surface antigens (CD105 + , CD90 + , CD73 + , CD14 − , CD20 − , CD34 − , CD45 − ) in the HCFs was analysed by flow cytometry using a MACSQuant Analyzer 10 flow cytometer (Cat# 130-096-343, Miltenyi Biotec, Bergisch Gladbach, Germany), MACSQuantify software version 2.13.3 (Miltenyi Biotec) and MSC Phenotyping Kit (Cat# 130-095-198, Miltenyi Biotec), following the manufacturer’s instructions.

Techniques: Flow Cytometry, Expressing, Control, Marker