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Effect of x-PVA/HA on <t>HaCaT</t> Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.
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Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Electrospun poly(vinyl alcohol)/hyaluronic acid nanofiber mats for wound care in space: cytocompatibility and effects of UV-C treatment and simulated microgravity

doi: 10.3389/fbioe.2026.1946755

Figure Lengend Snippet: Effect of x-PVA/HA on HaCaT Cell Viability. (a) Confocal microscopy orthogonal projection images showing dead cells (PI + , red fluorescence), total cells (PKH67, green fluorescence), and merged images (PI + /PKH67) under control, adherent, and spheroid-like conditions after 24 h of exposure to x-PVA/HA (20× objective; scale bar = 20 μm). (b) Graphical representation of the ratio between the sum of red fluorescence intensity (PI + dead cells) and the sum of green fluorescence intensity (PKH67, total cells) in control (8.54 ± 1.43) and x-PVA/HA-exposed adherent cells (7.56 ± 0.67). Data are presented as mean ± SEM. No statistically significant difference in the proportion of dead cells was observed between the two conditions (Student’s t-test: control vs. x-PVA/HA adherent cells, p > 0.05; ns, not significant). x-PVA/HA-exposed spheroid-like cells showed a low number of PI + dead cells; however, these values were not quantitatively compared with the control condition because control cells cultured on plastic did not form spheroid-like structures.

Article Snippet: NT2D1 human embryonal carcinoma cells, HEK293 human embryonic kidney cells, and HaCaT human keratinocytes were obtained from the American Type Culture Collection (ATCC, Manassas, VA, United States).

Techniques: Confocal, Microscopy, Fluorescence, Control, Cell Culture