Journal: Current Issues in Molecular Biology

Article Title: In Vitro Digestive-Enzyme Inhibition and Antiglycation Activity of Cannabis sativa L. Essential Oil: Experimental Evaluation and Molecular Docking Analysis

doi: 10.3390/cimb48090938

Figure Lengend Snippet: Inhibitory effect of CSEO and Gallic acid (positive control) on glycation activity of hemoglobin. Results are shown as mean ± SEM ( n = 3). Graphs marked by different letters (a, b, c) are significantly different (the significance started at p < 0.05).

Article Snippet: Advanced glycation end-product (AGE) formation was quantified by fluorescence spectroscopy (excitation 355 nm, emission 460 nm; Horiba FluoroMax-4, Kyoto, Japan).

Techniques: Positive Control, Activity Assay

Journal: Current Issues in Molecular Biology

Article Title: In Vitro Digestive-Enzyme Inhibition and Antiglycation Activity of Cannabis sativa L. Essential Oil: Experimental Evaluation and Molecular Docking Analysis

doi: 10.3390/cimb48090938

Figure Lengend Snippet: IC 50 values of the multiple stages of albumin glycation. Data are presented as mean ± SEM ( n = 3).

Article Snippet: Advanced glycation end-product (AGE) formation was quantified by fluorescence spectroscopy (excitation 355 nm, emission 460 nm; Horiba FluoroMax-4, Kyoto, Japan).

Techniques: Inhibition

Journal: Frontiers in Nutrition

Article Title: Curcumin attenuates liver injury by modulating the AGE–RAGE axis and metabolic homeostasis in high-fat diet/streptozotocin-induced type 2 diabetic mice

doi: 10.3389/fnut.2025.1710380

Figure Lengend Snippet: CUR modulates AGE/RAGE signaling and its downstream targets PI3K/Akt and NFκB pathways. (A,B) The levels of plasma (A) and hepatic AGEs (B) were measured by ELISA ( n = 5). (C) The mRNA expression levels of RAGE in the liver were analysed ( n = 3). (D–F) Representative bands and quantitative expression levels of p-PI3K, PI3K, p-Akt, Akt, p-NFκB p65, and NFκB p65 proteins in the liver from Western blot were presented. β-actin was served as an internal reference ( n = 3). For quantitative analysis (D–F) , the band intensity of each target protein was normalized to its corresponding internal control. The normalized values were presented relative to the mean of the NC group, which was set to 1. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant difference.

Article Snippet: The AGEs levels in the plasma and liver tissues were determined using a mouse-specific ELISA kit (CUSABIO Biotech, Wuhan, China; CAT#CSB-E09414m) according to the manufacturer’s protocol.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control