Journal: Journal of Nuclear Medicine

Article Title: PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer

doi: 10.2967/jnumed.125.270922

Figure Lengend Snippet: (A) Representative fluorescent images of MOC1 and MOC2 cells after 48 h of incubation with increasing titers of GFP-expressing RP1-15. GFP shown in gray scale. (B) MOC1 and MOC2 cell viability after 48 h with increasing RP1 titers in vitro, assessed via CellTiter-Glo assay. Significance determined using 1-way ANOVA with Dunnett test. RLU = relative light units.

Article Snippet: The MOC2( PD-L1 ) cell line was generated in our laboratory to overexpress PD-L1 by transducing MOC2 cells with a lentiviral vector carrying the murine CD274 gene tagged with a green fluorescent protein (GFP) reporter (MR 203953L2; OriGene Technologies).

Techniques: Incubation, Expressing, In Vitro, Glo Assay

Journal: Synthetic and Systems Biotechnology

Article Title: AmXlnR, a transcription factor involved in xylan degradation and pentose catabolism, enhances pullulan production from xylose in Aureobasidium melanogenum

doi: 10.1016/j.synbio.2026.02.007

Figure Lengend Snippet: In vivo G FP expression analysis control of the native and mutated promoters of AmXLN2 , AmBXL2 and AmABF1 . ( a ) Schematic representation of the native promoters and their variants with deleted putative XlnR-binding sites: P XLN2 Δ:P XLN2 with 5′-GGCTGA-3′ deleted; P BXL2 Δ:P BXL2 with 5′-GGTTAA-3′ deleted; P ABF1 Δ:P ABF1 with 5′-GGCTAT-3′ deleted. ( b ) Relative fluorescence intensity, ( c ) relative GFP transcriptional level, and ( d ) bright-field and corresponding fluorescence images of the reporter strains. Three independent replicates were performed for the statistical analysis. ∗ P < 0.05,∗∗ P < 0.01, ns: no significance.

Article Snippet: GFP fluorescence intensity was visualized using an Olympus U-LH100HG fluorescent microscope and quantified using a BioTeK Synergy H1 Hybrid Reader (BioTek Instruments Inc., USA) (485 nm excitation and 520 nm emission).

Techniques: In Vivo, Expressing, Control, Binding Assay, Fluorescence