Journal: Neural Regeneration Research

Article Title: Blood–brain barrier disruption and neuroinflammation in the hippocampus of a cardiac arrest porcine model: Single-cell RNA sequencing analysis

doi: 10.4103/NRR.NRR-D-24-01269

Figure Lengend Snippet: Single-cell atlas of hippocampus in cardiac arrest and resuscitation pig model. (A) Schematic representation of the experimental procedure to induce ventricular fibrillation by electrocution, followed by cardiopulmonary resuscitation, in pigs. Hippocampal tissue harvested from euthanized animals was dissociated and subjected to scRNA-seq. (B) Representative images of TUNEL staining and quantification of the percentage of TUNEL-positive cells ( n = 3 pigs; 2–3 slices per pig). Values presented as means ± standard deviation. Statistical analysis was performed using one-way analysis of variance followed by Tukey’s post hoc test; **** P < 0.0001. (C) UMAP visualization of scRNA-seq data from the hippocampus, showing 26 distinct clusters. (D) Ten distinct cell types identified in the hippocampus. (E) Pie chart showing the proportions of different cell types identified across all samples. (F) Bar graphs of cell type proportions displayed by cell type (left) and by group (right). (G) Box plots showing the relative proportions of various cell types between the ROSC6h vs . Sham groups (left) and the ROSC24h vs. Sham groups (right), as calculated by the scCODA model. ROSC: Return of spontaneous circulation; scRNA-seq: single-cell RNA sequencing; TUNEL: TdT-mediated dUTP nick end labeling UMAP: Uniform Manifold Approximation and Projection.

Article Snippet: The sections were deparaffinized through a series of graded ethanol and clearing solutions, rehydrated in distilled water, and then stained using a TdT-mediated dUTP nick end labeling (TUNEL) assay kit (Servicebio, Wuhan, China, Cat# G1507) in accordance with the manufacturer’s instructions.

Techniques: TUNEL Assay, Staining, Standard Deviation, RNA Sequencing, End Labeling