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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual <t>respiratory</t> specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).
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Evaluation of lyo-cLAMP with residual respiratory specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).

Journal: The Analyst

Article Title: Lyophilised colourimetric LAMP for visual readout with dual colour indicators

doi: 10.1039/d5an01238d

Figure Lengend Snippet: Evaluation of lyo-cLAMP with residual respiratory specimens from nasal, nasopharyngeal or buccal swabs. Pictures of lyo-cLAMP reactions after 35 min incubation at 63 °C using: (A) lyo-cLAMP MM1 (phenol red 100 µM), (B) lyo-cLAMP MM2 (phenol red 100 µM with eriochorme black T 75 µM), (C) lyo-cLAMP MM3 (thymol blue 150 µM with eriochorme black T 100 µM), (D) lyo-cLAMP MM4 (bromothymol blue 100 µM with eriochorme black T 75 µM), and (E) lyo-cLAMP MM5 (bromothymol blue 100 µM with calmagite 50 µM). (F) PCR C q values distribution using CDC assays targeting SARS-CoV-2 (N1). Extracted nucleic acids from nasal, nasopharyngeal or buccal swabs ( N = 15).

Article Snippet: Analytical specificity of the assay was evaluated experimentally using the Twist Respiratory Virus Research Panel (Cat no. 103730, Twist Bioscience) (Fig. S1).

Techniques: Incubation