Journal: eLife

Article Title: Specific GPCRs elicit unique extracellular vesicle miRNA array signatures

doi: 10.7554/eLife.107865

Figure Lengend Snippet: ( A ) Dose-dependent cAMP accumulation after ADORA1 activation with agonist, 2-chloro- N 6 -cyclopentyladenosine (CCPA), or inhibition with antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) (5 μM). ( B ) Dose-dependent IP1 accumulation after 2-pyridylethylamine dihydrochloride (PEA) stimulation of histamine receptor H1 (HRH1) and inhibition by cetirizine (1 μM). ( C ) Alkaline phosphatase (ALP) activity after stimulation of frizzled class receptor 4 (FZD4) by Wnt3a. ( D ) The ratio of phosphorylated ERK1/2 (pERK1/2) by total ERK 1/2 (tERK1/2) after stimulation of ACKR3 by SDF-1α. For all graphs, data are shown as mean ± SD, with n=3 independent repeats, each having duplicate determinants, ns = not significant, *p<0.05, **p<0.01, ***p<0.001 vs. vehicle control (VC). Statistical significances were determined by one-way or two-way ANOVA of receptors by agonists or antagonists and post hoc Tukey’s or Sidak’s testing for multiple comparisons.

Article Snippet: Chemical compound, drug , 2-Chloro- N 6 -cyclopentyladenosine (CCPA) , Tocris Bioscience , 1705 CAS:37739-05-2 , .

Techniques: Activation Assay, Inhibition, Activity Assay, Control

Journal: eLife

Article Title: Specific GPCRs elicit unique extracellular vesicle miRNA array signatures

doi: 10.7554/eLife.107865

Figure Lengend Snippet: U2OS cells were incubated with VC or a selective agonist 2-chloro- N 6 -cyclopentyladenosine (CCPA) ( A ), 2-pyridylethylamine dihydrochloride (PEA) ( B ), Wnt3a ( C ), or SDF-1α ( D ) for ADORA1, HRH1, FZD4, and ACKR3, respectively. EVs were isolated, and the concentration was normalized as a percentage of the media control (MC). EV size distribution following agonist or VC treatment in ADORA1 ( E ), HRH1 ( F ), FZD4 ( G ), and ACKR3 ( H ). Data are shown as mean ± SEM, n=3. A t-test determined no significant differences in EV concentration or size distribution between the VC and agonist stimulation in all G protein-coupled receptors (GPCRs), p>0.05.

Article Snippet: Chemical compound, drug , 2-Chloro- N 6 -cyclopentyladenosine (CCPA) , Tocris Bioscience , 1705 CAS:37739-05-2 , .

Techniques: Incubation, Isolation, Concentration Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Thalamic distribution and effects of 5-HT2C receptors on tonic GABAA inhibition and absence seizures: implications for treatment

doi: 10.3389/fphar.2026.1729460

Figure Lengend Snippet: Distribution of 5-HT 2C Rs in the VB and NRT. Brightfield photomicrographs showing the distribution of 5-HT 2C receptor immunoreactivity in the VB and NRT (A,D,G) , and enlarged sections of the NRT (B,E,H) and VB (C,F,I) of P25-30 NEC, GAERS and Wistar rats, as indicated. (J,K) Quantification of 5-HT 2C R + neuron density in the NRT and VB of the three strains. No significant differences were observed in the NRT. In the VB, Wistar rats displayed a significantly higher 5-HT 2C R + neuron density than NEC and GAERS ( P < 0.05), with GAERS showing the lowest count ( P < 0.05). Scale bar = 200 µm in (G) (applies to (A,D,G) ) and = 50 µm in (I) (applies to (B,C,E,F,H,I) ). One-Way ANOVA with Tukey’s multiple post-test comparison (* P < 0.05).

Article Snippet: The 5-HT 2C R agonist (2S)-1-(6-chloro-5-fluoroindol-1-yl)propan-2-amine (Ro 60-0175), the 5-HT 2C R antagonist 6-chloro-5-methyl-N-(6-(2-methylpyridin-3-yl)oxypyridin-3-yl)-2 (SB242084) and gabazine (SR 95531 hydrobromide) were purchased from Tocris Bioscience (United Kingdom).

Techniques: Comparison