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Effects of different concentrations of RSPO1 and <t>CHIR99021</t> in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.
Chir99021, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of different concentrations of RSPO1 and <t>CHIR99021</t> in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.
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Effects of different concentrations of RSPO1 and <t>CHIR99021</t> in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.
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Effects of different concentrations of RSPO1 and <t>CHIR99021</t> in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.
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Effects of different concentrations of RSPO1 and <t>CHIR99021</t> in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.
Chir99021, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.

Journal: Poultry Science

Article Title: Establishment of a broiler jejunal organoid model based on optimized activators or optimized two-step media and its assessment

doi: 10.1016/j.psj.2026.106930

Figure Lengend Snippet: Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the differentiation of broiler JOs (experiment 1). A ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on differentiation of broiler JOs; B ) Effects of different concentrations of RSPO1 and CHIR99021 in JODM on the budding percentage of broiler JOs; C ) Effect of passage on the differentiation of broiler JOs; D ) Effect of cryopreservation on the differentiation of broiler JOs. RSPO1: R-spondin 1. JODM: jejunal organoid differentiation medium. JOs: jejunal organoids. Different letters (A, B, C) indicate significant differences among treatments ( P < 0.01). Data are means ± SE (n = 6). Scale bar = 100 μm.

Article Snippet: The components of JOGM were identical to CMDM, and the components of JODM contained 97% ( v / v ) Advanced DMEM/F12, 1% ( v / v ) P/S, 1% ( v / v ) Glutamax, 1% ( v / v ) HEPES, 1 mM NAC, 10 μM Y-27632 2HCl, 10 μM SB202190, 0.5 μM A83-01, 50 ng/mL EGF, 50 ng/mL RSPO1 (RC010070C, XianJue Biotechnology, Suzhou, China), 50 ng/mL Noggin (RC010100C, XianJue Biotechnology, Suzhou, China), and 2.5 μM CHIR99021 (HY-10182, MCE, NJ, USA).

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