Journal: Bioactive Materials

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

doi: 10.1016/j.bioactmat.2026.02.051

Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

Techniques: In Vitro, Fluorescence, Staining

Journal: Nucleic Acids Research

Article Title: Replication protein A protects lagging strand gaps, restricting PARP inhibitor-induced synthetic lethality in BRCA1-deficient tumors

doi: 10.1093/nar/gkag396

Figure Lengend Snippet: Chemical RPAi slows fork progression and inhibits replication restart in A549 NSCLC cells. ( A ) A549 cell proliferation was assessed via Incucyte live cell imaging as a function of NERx-329 and cisplatin treatment. The data are presented as the mean and SEM of triplicate determinations. ( B ) Replication fork progression in response to treatment with 30 µM NERx-329 was assessed by molecular combing using the scheme indicated. Data are presented as the median and individual values from at least 100 replication tracks from duplicate experiments. Representative tracks are indicated below the graph. Statistical analysis was performed via Student t -test **** P < .0001. ( C ) Replication fork restart in response to 4 mM HU, 50 µM mirin, and/or 30 µM NERx-329 was assessed by molecular combing using the scheme indicated. Data are presented as the median and individual values from at least 100 replication tracks from duplicate experiments. Representative tracks are indicated below the graph. Statistical analysis was performed via one way ANOVA (* P < .05, **** P < .0001).

Article Snippet: The Incucyte Cell Cycle Green/Red Lentivirus Reagent (Sartorius, Cat# 4779) was added at an MOI of 4.5 in media containing 8 μg/ml polybrene (Millipore Sigma, Cat# TR-1003).

Techniques: Live Cell Imaging

Journal: Nucleic Acids Research

Article Title: Replication protein A protects lagging strand gaps, restricting PARP inhibitor-induced synthetic lethality in BRCA1-deficient tumors

doi: 10.1093/nar/gkag396

Figure Lengend Snippet: FEN1 loss sensitizes A549 NSCLC cells to RPAi. ( A ) Western blot confirming KO of FEN1 in A549 clonal cell lines. ( B ) Incucyte live cell imaging analysis of indicated A549 cells treated with 2.5 µM NERx-329. The data are presented as the mean and SEM of triplicate determinations. ( C ) Analysis of viability via CCK-8 metabolic assay in the A549 NTC, FEN1 heterozygous, and homozygous KO cells after treatment with RPAi NERx-329. The IC50 values were calculated by nonlinear regression analysis.

Article Snippet: The Incucyte Cell Cycle Green/Red Lentivirus Reagent (Sartorius, Cat# 4779) was added at an MOI of 4.5 in media containing 8 μg/ml polybrene (Millipore Sigma, Cat# TR-1003).

Techniques: Western Blot, Live Cell Imaging, CCK-8 Assay, Metabolic Assay

Journal: Nucleic Acids Research

Article Title: Replication protein A protects lagging strand gaps, restricting PARP inhibitor-induced synthetic lethality in BRCA1-deficient tumors

doi: 10.1093/nar/gkag396

Figure Lengend Snippet: PARP1 specificity dictates PARPi - RPAi combination response. ( A ) Confluence of MDA-MB-436 cells treated with vehicle control, NERx-329, saruparib, or their combination was monitored by Incucyte live-cell imaging. ( B ) Cytotox Red dye fluorescence intensity from the experiment depicted in panel (A). ( C ) Confluence of UWB1.289 cells treated with the vehicle control, NERx-329, saruparib, or a combination, as monitored by Incucyte live-cell imaging. ( D ) Confluence of BRCA1-complemented UWB1.289 cells treated with vehicle control, NERx-329, saruparib, or a combination, as monitored by Incucyte live-cell imaging.

Article Snippet: The Incucyte Cell Cycle Green/Red Lentivirus Reagent (Sartorius, Cat# 4779) was added at an MOI of 4.5 in media containing 8 μg/ml polybrene (Millipore Sigma, Cat# TR-1003).

Techniques: Control, Live Cell Imaging, Fluorescence