Journal: Disease Models & Mechanisms

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1242/dmm.052528

Figure Lengend Snippet: Generation of the Cdx2 cre/+ ;Pax3 fl/fl mouse model. (A) Genetic cross used to generate mice with spina bifida (SB). Cdx2 cre drives Pax3 knockout in the body only (light-green highlight), whereas the head remains wild type. See Table 1 for offspring genotypes. (B,C) Cdx2 cre driving mTmG reporter expression in the body at embryonic day (E)10.5 (B; blue) and E15.5 (C; green). Magenta in B indicates a region of no Cdx2 cre -mediated recombination. Dashed line in C indicates outline of the fetus. See also Fig. S1 . (D,E) Representative images of E15.5 control ( Cdx2 +/+ ;Pax3 fl/fl ; D) and mutant ( Cdx2 cre/+ ;Pax3 fl/fl ; E) fetuses. White arrowheads indicate the location of the open SB lesion (in E). See also Fig. S2 . (F) Phenotypic developmental timeline of Cdx2 cre/+ ;Pax3 fl/fl embryos and fetuses, with dashed lines indicating the extent of open SB lesions. Scale bars: 1.0 mm.

Article Snippet: Cdx2 cre/+ male mice ( ) were obtained from The Jackson Laboratory [JAX; strain 009350 : B6.Cg-Tg(CDX2-cre)101Erf/J] and maintained by breeding with C57BL/6J females.

Techniques: Knock-Out, Expressing, Control, Mutagenesis

Journal: Disease Models & Mechanisms

Article Title: Chiari II brain malformation is secondary to open spina bifida

doi: 10.1242/dmm.052528

Figure Lengend Snippet: PAX3 expression in E10.5 control and Cdx2 cre/+ ;Pax3 fl/fl embryos. Sagittal cryosections of control ( n =3) and SB ( n =3) embryos, immunostained for PAX3 (yellow) and nuclear stained with DAPI (magenta). (A,B) Head sections: PAX3 expression is present in the neural tube (hindbrain, outlined by dashed lines) in both control (A) and SB (B) embryos (yellow arrowheads). (C,D) Lower body: PAX3 expression is present in the dorsal neural tube and dermomyotomes of the control spinal region (yellow arrowheads in C), but is not detectable in the SB embryo (D), consistent with knockout of Pax3 in the lower body. Bracket in D indicates spina bifida region. Scale bar: 0.5 mm.

Article Snippet: Cdx2 cre/+ male mice ( ) were obtained from The Jackson Laboratory [JAX; strain 009350 : B6.Cg-Tg(CDX2-cre)101Erf/J] and maintained by breeding with C57BL/6J females.

Techniques: Expressing, Control, Staining, Knock-Out