Journal: Molecular Therapy Oncology

Article Title: Engineered Salmonella -mediated c-di-AMP delivery activates STING to remodel the tumor microenvironment

doi: 10.1016/j.omton.2026.201185

Figure Lengend Snippet: Effect of SL disA therapy on T cells and macrophage from tumor samples (A, B) Flow Cytometry analysis of CD3 and CD8 surface markers(A) and CD3 + CD8 + cells statistical graph (B). (C, D) Flow cytometry analysis of CD3 and CD4 surface markers (C) and CD3 + CD4 + cells statistical graph (D). (E, F) Flow cytometry analysis of F4/80 and CD86 surface markers (E) and F4/80 + CD86 + cells statistical graph (F). Data are expressed as mean ± SEM, n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001, one-way ANOVA with Tukey’s multiple comparisons tests.

Article Snippet: The following antibodies were used: FITC anti-mouse F4/80 (clone CI: A3-1), APC anti-mouse CD86 (clone GL-1), FITC anti-mouse CD3 (clone 17A2), APC anti-mouse CD4 (clone GK1.5), and APC anti-mouse CD8 (clone YTS-169), all purchased from Elabscience Biotechnology Co., Ltd.

Techniques: Flow Cytometry

Journal: Bioactive Materials

Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

doi: 10.1016/j.bioactmat.2025.12.055

Figure Lengend Snippet: In vitro cytocompatibility and immunomodulatory effects of different samples on macrophages. (A) Representative images of RAW264.7 cells cultured for 48 h on different substrates, stained for actin filaments (red) and nuclei (blue). (B) Immunofluorescent staining of M1 marker CD86 and M2 marker CD206 after 48 h under LPS stimulation. (C–D) Quantitative analysis of CD86 and CD206 fluorescence intensity. (E–H) Secretion levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by ELISA after 1 and 3 days of culture. (I–L) Relative mRNA expression levels of TNF- α , IL-1 β , IL-6, and IL-10 determined by RT-qPCR. Error bars represent means ± SD for n = 6, ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

Techniques: In Vitro, Cell Culture, Staining, Marker, Fluorescence, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR

Journal: Bioactive Materials

Article Title: Shikonin-loaded porous graphdiyne nanofilm on titanium surface for enhanced antibacterial activity and osseointegration

doi: 10.1016/j.bioactmat.2025.12.055

Figure Lengend Snippet: In vivo evaluation of osteogenesis and osseointegration. (A) IHC staining of CD86 in peri-implant tissue at 7 and 14 days. (B) Quantitative analysis of CD86-positive staining areas. (C) H&E staining and (E) Masson's trichrome staining of the bone-implant interface at four weeks. (D) Quantification of new bone formation area based on histological sections. (F) Bone-to-implant contact percentage determined from Masson's trichrome staining. Error bars represent means ± SD for n = 4, ∗ p < 0.05, ∗∗ p < 0.01.

Article Snippet: The antibodies of CD86 and CD206 were purchased from Sanying Biotechnology Co., Ltd. (Wuhan, China).

Techniques: In Vivo, Immunohistochemistry, Staining