Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a , b TEM images of P-CD@MM. c Measurements of the expression levels of CD86 and CD206 in each group. d Representative fluorescence images of cellular uptake of P-CD@MM in 4T1 or NIH-3T3. e , f Relative cell viability of NIH-3T3 and 4T1 cells after different treatments ( n = 6 independent samples). g , h Live/dead cell and ROS staining of 4T1 cells after different treatments. For ( a – d , g , h ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via two-tailed Student’s t test. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Expressing, Fluorescence, Staining, Two Tailed Test

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a Confocal images of CRT exposure in 4T1 cells after different treatments. b , c Evaluation of HMGB1 and ATP levels in 4T1 cells after different treatments ( n = 6 independent samples). d A scheme to show the experimental process of the evaluation of DC maturation (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). e , f Evaluation of DC maturation level in each group ( n = 6 mice). g – i Flow cytometry analysis and the corresponding quantitative results of CD86 + and CD206 + expression in TAMs of BMDM after different treatments ( n = 6 mice). For ( a ), experiment was repeated three times independently with similar results. Data are presented as mean ± SD and statistical significance was analyzed via one-way ANOVA. Source data are provided as a file.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Flow Cytometry, Expressing

Journal: Nature Communications

Article Title: D-π-A sensitized carbon dots as long-lived type-I/Ⅱ photosensitizers for NIR-excited hypoxia-regulated photodynamic therapy

doi: 10.1038/s41467-026-71476-y

Figure Lengend Snippet: a CRT staining of tumor tissues after different treatments ( n = 3 mice). b Expression levels of CD4 + T cells and CD8 + T cells in the primary tumors after different treatments ( n = 6 mice). c Expression levels of CD86 + and CD206 + in TAMs in the primary tumors after different treatments ( n = 6 mice). d Schematic diagram of P-CD@MM-mediated PDT to induce an immune response in vivo (Relative drawing materials were created in BioRender. zhenlin, Z. (2026) https://BioRender.com/9u519d6 ). For ( a ), experiment was repeated three times independently with similar results.

Article Snippet: The membrane was incubated overnight at 4 °C with primary antibodies: anti-β-actin Rabbit mAb (ABclonal, AC026, 1:5000), anti-CD206 Rabbit mAb (ABclonal, A26948, 1:5000), and anti-CD86 Rabbit mAb (ABclonal, A21198, 1:10000).

Techniques: Staining, Expressing, In Vivo