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cd47 detection  (Miltenyi Biotec)
93
Miltenyi Biotec cd47 detection
Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human <t>CD47</t> gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
Cd47 Detection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd47+detection/CD47+Antibody%2C+anti-human%2C+REAfinity/pmc09226073-361-1-9
Average 93 stars, based on 1 article reviews
cd47 detection - by Bioz Stars, 2026-10
93/100 stars
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mouse anti-human cd47-fitc detection kit  (Thermo Fisher)
90
Thermo Fisher mouse anti-human cd47-fitc detection kit
Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human <t>CD47</t> gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).
Mouse Anti Human Cd47 Fitc Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd47+detection/anti+cd47+antibody+14+0479+82+clone+b6h12/pmc05774548-161-21-30
Average 90 stars, based on 1 article reviews
mouse anti-human cd47-fitc detection kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).

Journal: Nature Communications

Article Title: Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

doi: 10.1038/s41467-022-31386-1

Figure Lengend Snippet: Schematic presentation of different parallel or anti-parallel coiled coil pairing. N5N6 (strong parallel CC pair), P3AP4 (anti-parallel CC pair) and P3SP4S (weak parallel CC pair) ( a ). Diagram showing gRNA positions within exon 2 of the human CD47 gene ( b ). Indel mutation quantification by the T7E1 assay, determined 48 h post transfection (HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding gRNA and CCExo) for several tested gRNAs ( c – h ), a combination of all gRNAs ( i ), and gRNA1 in combination with CCExo containing coiled-coils with increasing affinity (P3P4 < N5N6) ( j ). Data present three individual separate experiments ( n = 3). * P < 0.05, **< 0.01, ***< 0.001 and **** P < 0.0001. All P values are from ordinary one-way ANOVA followed by Tukey’s multiple comparisons test compared to Cas9 values or if stated otherwise by brackets. Data are presented as mean values ± SEM as appropriate ( c – j ). Expression of Cas9 or its variants in HEK293 cells 48 h post transfection determined by western blot and immunodetection using specific anti-Cas9 antibodies. α-tubulin was used as a loading control. A representative blot from two individual separate experiment is shown ( k ).

Article Snippet: For CD47 detection we used human CD47-FITC antibodies from Miltenyi Biotec (130-101-344).

Techniques: Mutagenesis, Transfection, Expressing, Western Blot, Immunodetection

HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).

Journal: Nature Communications

Article Title: Coiled-coil heterodimer-based recruitment of an exonuclease to CRISPR/Cas for enhanced gene editing

doi: 10.1038/s41467-022-31386-1

Figure Lengend Snippet: HEK293 cells (2*10 5 cells/ml) were transfected with plasmids encoding target gRNA and CCExo. Genomic DNA was isolated 48 h post transfection, the targeted region PCR amplified and subjected to NGS analysis. NGS analysis was performed for 200 bp amplicons for gRNA targeting MYD88 gene ( a ) or 400 bp amplicons for gRNA targeting CD47 gene ( b ), VEGFα gene ( c ) or EMX1 gene ( d ). Diagrams are showing percentage (calculated based on number of total mutated reads) of deletions (Δ) of various lengths. Genome wide determinations of potential off-target activities of CCExo for VEGFα sites in HEK293 cells and for TRBC1 site in human K562 cells, predicted by CIRCLE-seq ( e ).

Article Snippet: For CD47 detection we used human CD47-FITC antibodies from Miltenyi Biotec (130-101-344).

Techniques: Transfection, Isolation, Amplification, Genome Wide