Journal: Frontiers in Immunology

Article Title: A novel Bacillus megaterium /ω-3 PUFA synbiotic attenuates colon inflammation and promotes a pro-resolving macrophage profile ex-vivo

doi: 10.3389/fimmu.2026.1916994

Figure Lengend Snippet: Representative images of immunofluorescence staining with CD68 and CD163 of human colon sections obtained from healthy patients in basal condition (A) , treated for 24 hours with 2,5 μL of SynΩ3 formulation (B) , or with ω-3 lysine salt dispersion alone (C) . DAPI-stained nuclei are in blue. Scale bar: 100μm; zoom inset up: 50μm; zoom inset down: 50μm. (D) The bar graph shows the CD68 and CD163 co-localization rate of human colon sections obtained from IBD patients in basal condition treated for 24 hours with 2,5 μL of SynΩ3 formulation or with ω-3 lysine salt dispersion alone. n= 2.

Article Snippet: Colon sections were incubated overnight at 4 °C with the following primary antibodies: mouse monoclonal anti-CD68 antibody (1:50, Abcam, catalog ab955, clone KP1), rabbit polyclonal anti-CD163 antibody (1:50 ABclonal, catalog A8383), and mouse monoclonal anti-CD206 antibody (1:50, Proteintech, catalog 60143-1).

Techniques: Immunofluorescence, Staining, Formulation, Dispersion

Journal: Frontiers in Immunology

Article Title: A novel Bacillus megaterium /ω-3 PUFA synbiotic attenuates colon inflammation and promotes a pro-resolving macrophage profile ex-vivo

doi: 10.3389/fimmu.2026.1916994

Figure Lengend Snippet: Representative images of immunofluorescence staining with CD68 and CD163 of human colon sections obtained from UC patients in basal condition (A) , treated for 24 hours with 2,5 μL of SynΩ3 formulation (B) or with ω-3 lysine salt dispersion alone (C) . DAPI-stained nuclei are in blue. Scale bar: 100μm; zoom inset up: 50μm; zoom inset down: 50μm. (D) The bar graph shows the CD68 and CD163 co-localization rate of human colon sections obtained from UC patients in basal condition treated for 24 hours with 2,5 μL of SPMs generated by SynΩ3 formulation or with ω-3 lysine salt dispersion alone. n= 3.

Article Snippet: Colon sections were incubated overnight at 4 °C with the following primary antibodies: mouse monoclonal anti-CD68 antibody (1:50, Abcam, catalog ab955, clone KP1), rabbit polyclonal anti-CD163 antibody (1:50 ABclonal, catalog A8383), and mouse monoclonal anti-CD206 antibody (1:50, Proteintech, catalog 60143-1).

Techniques: Immunofluorescence, Staining, Formulation, Dispersion, Generated

Journal: Bioactive Materials

Article Title: Immunomodulatory effects of biodegradable Mg–Cu–Zn alloy in esophageal cancer

doi: 10.1016/j.bioactmat.2026.02.046

Figure Lengend Snippet: Distribution of CD163 + M2 TAMs in AKR-derived allograft tumor tissues from immunocompetent C57BL/6 mice. (a, b) Representative IHC staining images showing CD163 + M2 TAMs in the (a) peritumoral stroma and (b) tumor islets. Lower panels display higher-magnification views of the regions outlined by red dashed boxes. (c, d) Quantification of CD163 + cells in the (c) peritumoral stroma and (d) tumor islets. (e) Comparison of CD163 + cell density between the peritumoral stroma and tumor islets. (f) Total number of CD163 + cells in allograft tumors (peritumoral stroma and tumor islets combined). (g, h) Comparison of the density between iNOS + cells and CD163 + cells in the (g) peritumoral stroma and (h) tumor islets. (i, j) Quantification of iNOS + /CD163 + ratio in the (i) peritumoral stroma and (j) tumor islets. p < 0.05 (∗), p < 0.01 (∗∗), p < 0.001 (∗∗∗). A field of view is ∼0.086 mm 2 in (c−j).

Article Snippet: Tissue sections were then incubated with primary antibodies against iNOS (22226-1-AP, ProteinTech, China), CD163 (A26411PM, Abclone, China), CD8 (SP16, Maixin, China), CD4 (SP35, Maixin, China) or Ki-67 (12202S, Cell Signaling Technology) for 12 h at 4 °C, followed by secondary antibodies (Beyotime Biotechnology, Nantong, China).

Techniques: Derivative Assay, Immunohistochemistry, Comparison