Journal: Frontiers in Neurology

Article Title: Increased expression of cell adhesion molecules in myofasciitis

doi: 10.3389/fneur.2023.1113404

Figure Lengend Snippet: Intensity of immunohistochemistry staining of cell adhesion molecules and their receptors expression in myofasciitis and HC.

Article Snippet: The immunohistochemical (IHC) analysis of cells expressing CD3 (1:200, MAC1477, Bio-Rad), CD4 (1:50, ab133616, Abcam), CD8 (1:50, ab93278, Abcam), CD68 (1:50, ab201340, Abcam), VCAM-1 (1:200, 11,444-1-AP, Proteintech), ICAM-1 (1:200, 10,831-1-AP, Proteintech), VLA-4 (1:200, 19,676-1-AP, Proteintech), CD11a/CD18 (1:1000, ab52895, Abcam), CD31/PECAM-1 (1:2000, ab9498, Abcam), and CD146 (1:200, Ab75769, Abcam) were performed using a 3, 3′-diaminobenzidine Detection Kit; the sections were then counterstained with hematoxylin.

Techniques: Immunohistochemistry, Staining, Expressing

Journal: Frontiers in Neurology

Article Title: Increased expression of cell adhesion molecules in myofasciitis

doi: 10.3389/fneur.2023.1113404

Figure Lengend Snippet: The expression of cell adhesion molecules in controls and patients with myofasciitis. (A,B) The weak expression of VCAM-1 in controls and up-regulated VCAM-1 on blood vessels and a few inflammatory cells in Patient 3. A and B, scale bar, 50 μm. (C,D) No positive staining of VLA-4 in controls and the expression of VLA-4 on scattered inflammatory cells in Patient 2. C and D, scale bar, 100 μm. (E,F) The low-level expression of ICAM-1 on vascular endothelium cells in controls and overexpressed ICAM-1 on vascular endothelial cells and a few inflammatory cells in Patient 2. E and F, scale bar, 50 μm. (G,H) No positive staining of CD11a/CD18 in controls and the expression of CD11a/CD18 on scatter inflammatory cells in Patient 3. G and H, scale bar, 100 μm. (I,J) The expression of PECAM-1 on blood vessels in controls and up-regulated PECAM-1 on blood vessels in Patient 3, especially in actively involved areas. (I , G) , scale bar, 100 μm. ( K,L) Very weak positive staining of CD146 on vascular endothelium cells in controls and strong intensity of CD146 on blood vessels in Patient 2. (K,L) , scale bar, 100 μm.

Article Snippet: The immunohistochemical (IHC) analysis of cells expressing CD3 (1:200, MAC1477, Bio-Rad), CD4 (1:50, ab133616, Abcam), CD8 (1:50, ab93278, Abcam), CD68 (1:50, ab201340, Abcam), VCAM-1 (1:200, 11,444-1-AP, Proteintech), ICAM-1 (1:200, 10,831-1-AP, Proteintech), VLA-4 (1:200, 19,676-1-AP, Proteintech), CD11a/CD18 (1:1000, ab52895, Abcam), CD31/PECAM-1 (1:2000, ab9498, Abcam), and CD146 (1:200, Ab75769, Abcam) were performed using a 3, 3′-diaminobenzidine Detection Kit; the sections were then counterstained with hematoxylin.

Techniques: Expressing, Staining

Journal: Frontiers in Neurology

Article Title: Increased expression of cell adhesion molecules in myofasciitis

doi: 10.3389/fneur.2023.1113404

Figure Lengend Snippet: Western blot of cells adhesion molecules in muscle biopsies from controls and patients with myofasciitis. (A) Representative Western blot of ICAM-1, CD11a/CD18, VCAM-1, VLA-4, PECAM-1, and CD146 in muscle biopsies for five controls and four patients, including Patient 1, Patient 2, Patient 3, and Patient 5. (B) Quantitative analysis of ICAM-1, CD11a/CD18, VCAM-1, VLA-4, PECAM-1, and CD146 relative expression. n = 5 for controls, n = 4 for myofasciitis. * p < 0.05. Statistical analyses were performed by Mann–Whitney U test.

Article Snippet: The immunohistochemical (IHC) analysis of cells expressing CD3 (1:200, MAC1477, Bio-Rad), CD4 (1:50, ab133616, Abcam), CD8 (1:50, ab93278, Abcam), CD68 (1:50, ab201340, Abcam), VCAM-1 (1:200, 11,444-1-AP, Proteintech), ICAM-1 (1:200, 10,831-1-AP, Proteintech), VLA-4 (1:200, 19,676-1-AP, Proteintech), CD11a/CD18 (1:1000, ab52895, Abcam), CD31/PECAM-1 (1:2000, ab9498, Abcam), and CD146 (1:200, Ab75769, Abcam) were performed using a 3, 3′-diaminobenzidine Detection Kit; the sections were then counterstained with hematoxylin.

Techniques: Western Blot, Expressing, MANN-WHITNEY