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Miltenyi Biotec
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Journal: Materials Today Bio
Article Title: Biomimetic 3D-Bioprinted organoids of thymic epithelial tumors for translational drug screening and biomarker identification
doi: 10.1016/j.mtbio.2026.102878
Figure Lengend Snippet: (A-B) H&E staining of primary TC tissue and corresponding 3DP organoids.Scale bars: 50 μm (C) Immunofluorescence images of TC organoids stained for DAPI, CD56, CD117, and pan-CK. Scale bars: 50 μm (D-E) H&E staining of primary THYM tissue and corresponding 3DP organoids. Scale bars: 50 μm (F) Immunofluorescence images of THYM organoids stained for DAPI, P63, pan-CK, and TdT.Scale bars: 100 μm. (G) CNV profiles of TC-Tumor. (H)TC-3DP organoids (I) TC-Matrigel organoids. (J) CNV profiles of THYM-Tumor. (K) THYM-3DP organoids. (L)THYM-Matrigel organoids.
Article Snippet: The following primary antibodies were used: Pan-CK (# MA513203 , invitrogen) at a dilution of 1:100,
Techniques: Staining, Immunofluorescence
Journal: Frontiers in Immunology
Article Title: ACK2 antibody conditioning enhances adoptive transfer of hematopoietic progenitors to study central trained immunity in mice
doi: 10.3389/fimmu.2026.1735878
Figure Lengend Snippet: ACK2 antibody clearance from circulation and depletion of BM HSPCs. (A) Experimental design: C57BL/6 mice were injected i.p. with PBS or 500 μg of purified ACK2 antibody and peripheral blood was collected at days 3, 4, and 5 post-injection for the detection of ACK2 antibody in serum. Representative dot plots showing competition of serum-ACK2 with fluorescently labeled anti-c-Kit clone ACK2 for binding to Lin – c-Kit + BM cells, and corresponding boxplots showing median cell percentages and IQR with whiskers representing the lowest and highest values. (B) Experimental design: C57BL/6 mice were injected i.p. with PBS or 500 μg of purified ACK2 antibody and BM was collected at days 3, 4, and 5 post-injection for the detection of BM HSPCs. Representative dot plots showing Lin – c-Kit + cells labeled with anti-c-Kit clones 2B8 or 3C11 in PBS- and ACK2-injected mice, with boxplots showing median cell percentages and IQR with whiskers representing the lowest and highest values. Statistical significance was determined by non-parametric Kruskal-Wallis test with Dunn’s post hoc multiple comparisons (* P < 0.05; ** P < 0.01; *** P < 0.001). Dot plots shown are representative of individual samples. Data is a pool of two independent experiments from two independent ACK2 production batches. n = 3–8 mice per condition. Figure partially created in BioRender.com . Guiu, (A) (2026).
Article Snippet: c-Kit (CD117) , PE-Vio770 ,
Techniques: Injection, Purification, Labeling, Binding Assay, Clone Assay