Journal: Bioactive Materials

Article Title: Natural killer cell-inspired dendritic mesoporous rare-earth nanoparticles potentiate X-ray-triggered reactive oxygen generation for low-dose radiotherapy-radiodynamic therapy

doi: 10.1016/j.bioactmat.2026.02.011

Figure Lengend Snippet: In vitro targeted cell uptake and oxygen supply ability. (a) SDS-PAGE electrophoresis profiles and Western blot results of TSSI-Ce 6 C-DMTm, NKEV, and TSSI-Ce 6 C-DMTm@NKEV. (b) Scheme of the tumor-targeting mechanism of TSSI-Ce 6 C-DMTm@NKEV. (c) Western blot results of NK cells, NKEV, TSSI-Ce 6 C-DMTm@NKEV, and TSSI-Ce 6 C-DMTm to indicate the presence of DNAM-1 and NKG2D. (d) CLSM images and quantification analysis of MDA-MB-231 cells treated with TSSI-Ce 6 C-DMTm and TSSI-Ce 6 C-DMTm@NKEV for 1, 3, and 9 h. (f) Flow cytometry profiles and (g) CLSM images of MDA-MB-231 cells pretreated with different antibodies and treated with TSSI-Ce 6 C-DMTm@NKEV for 9 h. (h) CLSM images of MDA-MB-231 cells pretreated with H 2 O 2 and treated with different formulations via [Ru(dpp) 3 ]Cl 2 staining for hypoxia levels observation. ∗∗∗∗ p < 0.0001.

Article Snippet: Then, the membrane was blocked using 5% skim milk and incubated using primary antibody of anti -DNAM-1 (ABclonal, A23200), anti -NKG2D (Bioss, bs-0938R), anti -β-actin (Beijing Solarbio Science & Technology Co., Ltd.), anti-Na/K ATPase (Abcam, ab254025), respectively.

Techniques: In Vitro, SDS Page, Electrophoresis, Western Blot, Flow Cytometry, Staining

Journal: iScience

Article Title: Syncytin-a deficiency compromises murine sperm function by suppressing PRL/PGE2 and PI3K/AKT/mTOR pathway

doi: 10.1016/j.isci.2026.115694

Figure Lengend Snippet: Syna CKO disrupts sperm morphology and ultrastructure (A) Sperm morphology analysis by the electron microscope. Scale bars, 20 μm. (B) Schematic diagram of normal mouse sperm structure. (C) HE staining of sperm. Scale bars, 10 μm and 40 μm. (D) Scanning electron micrographs of the sperm head and tail of WT and syna CKO mice. Scale bars, 4 μm and 5 μm. (E) The relative mRNA expressions of ENO4, AKAP3, CCDC39, SPAG6, syna, and synb genes. GAPDH was used as an internal control. Data are presented as mean ± SD of n = 3 biologically independent experiments. (F) The relative protein expressions of ENO4, AKAP3, CCDC39, SPAG6, syna, and synb genes. GAPDH was used as an internal control. Data are presented as mean ± SD of n = 3 biologically independent experiments. (G) Sperm immunofluorescence for syncytin-1 (red), PNA (green), and Dapi (blue). Scale bars, 10 μm. (H) Sperm immunofluorescence for mito-tracker (red) and Dapi (blue). Scale bars, 10 μm. (I) Sperm immunofluorescence for syncytin-b (green) and Dapi (blue). Scale bars, 5 μm. (J) Transmission electron micrographs of the sperm head. Scale bars, 0.5 μm and 1 μm. (K) Transmission electron micrographs of the sperm midpiece and principal piece. Scale bars, 0.8 μm and 1 μm. Mean ± standard error is displayed in dot plots and bar charts. ∗∗∗∗ p < 0.0001, ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05 by Student’s t test.

Article Snippet: Syncytin-1 , Bioss , Cat #bs-2962R; RRID: AB_11106881.

Techniques: Microscopy, Staining, Control, Immunofluorescence, Transmission Assay