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The potential function and pathways of TSF on DOX‐induced cardiotoxicity: (a) PPI network of 103 overlapping targets constructed based on the STRING database; (b) the PPI network map; (c) the molecular docking of HDAC8 with BRCA2.

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: The potential function and pathways of TSF on DOX‐induced cardiotoxicity: (a) PPI network of 103 overlapping targets constructed based on the STRING database; (b) the PPI network map; (c) the molecular docking of HDAC8 with BRCA2.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: Construct

TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunofluorescence

The mechanism diagram of TSF and Rg3 alleviating DOX‐induced myocardial injury by regulating the HDAC8/BRCA2/DRP1 pathway.

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: The mechanism diagram of TSF and Rg3 alleviating DOX‐induced myocardial injury by regulating the HDAC8/BRCA2/DRP1 pathway.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: