Journal: mSystems

Article Title: Distinct properties of human pathogenic Candida species revealed by systematic comparative phenotypic screening of clinical isolates

doi: 10.1128/msystems.00786-25

Figure Lengend Snippet: High-throughput phenotypic analysis. Screening steps ( A ). Pipetting steps were performed using a liquid handling robot. Strains were cultivated in 96-well microplates with optical density readings (600 nm) in regular intervals. Data analysis and curve fitting steps were performed in R (tidyverse, FactoMineR, growthcurver) and further with PCA and hierarchical clustering. Density plot of growth rates of clinical C. glabrata isolates under ( B ) different temperatures and ( C ) different levels of osmotic stress. Population spread was determined by calculating mean absolute deviation for each condition ( D ).

Article Snippet: The cell suspension was then dispensed into 96-well round-bottom plates containing 100 μL YNB broth (1.7 g/L Yeast Nitrogen Base [MP Biomedicals, Germany], 2% dextrose) per well.

Techniques: High Throughput Screening Assay