Journal: Scientific Reports

Article Title: Human amniotic membrane modulates collagen production and deposition in vitro

doi: 10.1038/s41598-024-64364-2

Figure Lengend Snippet: Effect of DHACM and LHACM on collagen modifying enzymes. Fold change in gene expression of BMP1 ( a and d ), LOX ( b and e ) and TGM2 ( c and f ) in HDFs after 6 days in the presence of TGFβ1 cultured with macromolecular crowding and treated with DHACM and LHACM. * p < 0.05 versus TGFβ1 control and # p < 0.05 versus basal control using one-way ANOVA; n = 3. Western blot analysis of BMP1, LOX, TGase2 in cell lysates ( g ) and LOX in cell culture supernatant ( h ). Full blots available in Supplementary Figures S and S .

Article Snippet: After blocking with 5% non-fat dry milk in 1× Tris Buffered Saline 0.05% Tween 20 for 1 h, membranes were probed with primary antibodies overnight at 4 °C: αSMA (19,245, Cell Signaling Technology, Danvers, MA), phospho-SMAD2 Ser465/467 (3108, Cell Signaling Technology, Danvers, MA), SMAD2 (3103 Cell Signaling Technology, Danvers, MA), phospho-SMAD3 Ser423/425 (ab40854, Abcam, Waltham, MA), SMAD3 (ab40854, Abcam, Waltham, MA), BMP1 (ab38953, Abcam, Waltham, MA), LOX (ab174316, Abcam, Waltham, MA), TGase2 (ab2386, Abcam, Waltham, MA), and GAPDH (ab8245, Abcam, Waltham, MA).

Techniques: Expressing, Cell Culture, Control, Western Blot