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Image Search Results


Primer and Taqman probe sequences used to amplify bisulphite converted DNA in MethyLight analysis

Journal: Clinical Epigenetics

Article Title: SOCS1 methylation level is associated with prognosis in patients with acute-on-chronic hepatitis B liver failure

doi: 10.1186/s13148-023-01495-9

Figure Lengend Snippet: Primer and Taqman probe sequences used to amplify bisulphite converted DNA in MethyLight analysis

Article Snippet: MethyLight was performed on Lightcycler 2.0 (Roche, Basel, Switzerland) following the standard protocol provided by manufacturer [ , ]: 95 °C for 10 min, then 50 cycles of 95 °C for 15 s and 60 °C for 1 min. Universal methylated and bisulphite- converted human control DNA (QIAGEN, Hilden, Germany) served as a methylated reference and Alu-C4 was used as a control reaction to normalise for input DNA.

Techniques:

( A ) Agarose gel electrophoresis of genomic DNA isolated from PBMCs of the study subjects. Lane 1 and 2 represent genomic DNA sample from CAD patients. Lane 4 and 5 represent genomic DNA sample from control subjects. 200 ng of genomic DNA was loaded in each well. ( B ) Agarose gel electrophoresis of bisulphite-converted genomic DNA. Lane 1, 2 and 3 represent bisulphite-modified DNA of CAD patients. Lane 5, 6 and 7 represent bisulphite-modified DNA of control subjects.

Journal: Scientific Reports

Article Title: Aberrant DNA methylation of M1-macrophage genes in coronary artery disease

doi: 10.1038/s41598-018-38040-1

Figure Lengend Snippet: ( A ) Agarose gel electrophoresis of genomic DNA isolated from PBMCs of the study subjects. Lane 1 and 2 represent genomic DNA sample from CAD patients. Lane 4 and 5 represent genomic DNA sample from control subjects. 200 ng of genomic DNA was loaded in each well. ( B ) Agarose gel electrophoresis of bisulphite-converted genomic DNA. Lane 1, 2 and 3 represent bisulphite-modified DNA of CAD patients. Lane 5, 6 and 7 represent bisulphite-modified DNA of control subjects.

Article Snippet: A 20 μL reaction mix consisted of 20 ng of bisulphite modified control DNA standards and a final concentration of: 1x MeltDoctor HRM Master Mix (Applied Biosystems, Life Technologies), 0.25 μM forward primer, 0.25 μM reverse primer and deionized water.

Techniques: Agarose Gel Electrophoresis, Isolation, Control, Modification