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FRA alleviates THR-induced pyroptosis in MLE-12 cells through specific binding to HIF-1α. (A) Western blot analysis of pyroptosis-related proteins following HIF-1α silencing under various treatments. (B) The pull down assay between FRA and HIF-1α by <t>using</t> <t>biotin-labeled</t> FRA <t>(Biotin-FRA).</t> (C) Molecular docking of FRA with HIF-1α. (D) Western blotting analysis of pyroptosis-related proteins following HIF-1α mutation under various treatments. Data are presented as mean ± SD (n = 6/group). #/*p<0.05, ##/**p<0.01, ns p>0.05. # versus negative control (NC) group, *versus NC-THR group, ns versus control group.
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FRA alleviates THR-induced pyroptosis in MLE-12 cells through specific binding to HIF-1α. (A) Western blot analysis of pyroptosis-related proteins following HIF-1α silencing under various treatments. (B) The pull down assay between FRA and HIF-1α by using biotin-labeled FRA (Biotin-FRA). (C) Molecular docking of FRA with HIF-1α. (D) Western blotting analysis of pyroptosis-related proteins following HIF-1α mutation under various treatments. Data are presented as mean ± SD (n = 6/group). #/*p<0.05, ##/**p<0.01, ns p>0.05. # versus negative control (NC) group, *versus NC-THR group, ns versus control group.

Journal: Frontiers in Immunology

Article Title: Fraxinellone mitigates acute lung injury by targeting HIF-1α to suppress pyroptosis and inflammation

doi: 10.3389/fimmu.2026.1826165

Figure Lengend Snippet: FRA alleviates THR-induced pyroptosis in MLE-12 cells through specific binding to HIF-1α. (A) Western blot analysis of pyroptosis-related proteins following HIF-1α silencing under various treatments. (B) The pull down assay between FRA and HIF-1α by using biotin-labeled FRA (Biotin-FRA). (C) Molecular docking of FRA with HIF-1α. (D) Western blotting analysis of pyroptosis-related proteins following HIF-1α mutation under various treatments. Data are presented as mean ± SD (n = 6/group). #/*p<0.05, ##/**p<0.01, ns p>0.05. # versus negative control (NC) group, *versus NC-THR group, ns versus control group.

Article Snippet: Biotin-labeled fraxinellone (Biotin-FRA) was synthesized by conjugating biotin to FRA via a PEG4 linker (MedChemExpress, Shanghai, China).

Techniques: Binding Assay, Western Blot, Pull Down Assay, Labeling, Mutagenesis, Negative Control, Control