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Medicago arachis hypogaea lectin (pna)
Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus <t>),</t> <t>PNA</t> (from Arachis <t>hypogaea</t> ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.
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Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures on total hemocytes from Cherax quadricarinatus by immunofluorescence and flow cytometry using lectin staining. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ). Representative scatter plot shows internal complexity (side scatter, SSC) and positive for lectin staining. Immunofluorescences are representative for each lectin staining. Scale bar: 10 µm.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Immunofluorescence, Flow Cytometry, Staining

Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Identification of glycan structures by subpopulation of hemocytes from Cherax quadricarinatus by Flow cytometry using lectin staining. Value in each histogram represent mean of percentage of three independent experiments. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa ), ALL (from Amaranthus lycocarpus ), SNA-I (from Sambucus nigra ), MAA-I ( Maackia amurensis ). Red lines represent control assays for specificity recognition using lectins incubated previously with 200 mM specific carbohydrates 30 min before the assays, blue lines are lectins without inhibition.

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Flow Cytometry, Staining, Control, Incubation, Inhibition

Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).

Journal: Comparative Immunology Reports

Article Title: Starch α-glucan microparticles trigger hemocyte activation in Cherax quadricarinatus crayfish

doi: 10.1016/j.cirep.2025.200234

Figure Lengend Snippet: Glycan patterns by subpopulation hemocytes after immunostimulation. raw starch microparticles (SMP), Vibrio parahaemolyticus (Vp) and Escherichia coli (Ec). Value in each heatmap represent mean of percentage of three independent experiments. Significant differences are mentioned in the text. ConA (Concanavalin A from Canavalia ensiformis ), LTL (from Lotus tetragonolobus ), PNA (from Arachis hypogaea ), WGA (from Wheat Germ), VVA-I (from Vicia villosa), ALL (from Amaranthus lycocarpus ), SNA (from Sambucus nigra), MAA-I ( Maackia amurensis ).

Article Snippet: BA-7801–2, Newark, CA, USA); PNA from Arachis hypogaea (specificity: Gal β−1,3 GalNAc-Ser/Thr) (VectorLabs Inc, Cat Num.

Techniques: Glycoproteomics, Starch