Journal: Bioactive Materials

Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

doi: 10.1016/j.bioactmat.2026.05.005

Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression

Journal: Cancer Cell International

Article Title: Quantitative proteomic landscape reveals the role of CSPG5 in the recurrence of glioma

doi: 10.1186/s12935-026-04430-z

Figure Lengend Snippet: CSPG5 regulates ErbB2 stability via the lysosomal degradation pathway. ( A ) The heatmap shows the correlation of CSPG5 protein expression and ErbB family member activation in glioma of CPTAC dataset. Dot size = protein expression abundance. CPTAC grade distribution: 22 grade II, 30 grade III, 36 grade IV. ( B ) Linear regression analysis of CSPG5 and ErbB receptors protein expression levels. ( C ) qRT-PCR analysis of ErbB2 and ErbB3 in vector and CSPG5 overexpression GL261 cells. ( D ) CSPG5 overexpression and vector transfected GL261 cells were treated with 25 µg/mL of CHX for the indicated time points and ErbB2 protein levels were determined by Immunoblotting. Shown are ErbB2 levels normalized to α-tubulin and relative to 0 h timepoint. ( E ) Half-lives (T1/2) represent the time required for ErbB2 to decrease to 50% of its initial level. Data are shown as means ± SD from three independent experiments (* P < 0.05, ** P < 0.01, *** P < 0.001, ns: no significance, by two-way ANOVA test). ( F ) ErbB2 is primarily degraded through the lysosomal pathway. GL261 cells were treated with MG132 (10 µM) or chloroquine (50 µM) for 4 h to inhibit proteasome and lysosome activities. The expression of ErbB2 was analyzed by immunoblotting. ( G-H ) Confocal microscopy imaging of colocalization of ErbB2 (G) or ErbB3 (H) with lysosomes in vector and cspg5 overexpression GL261 cells

Article Snippet: Cells were fixed with 4% paraformaldehyde in PBS for 20 min, and then permeabilized with 0.1% Triton X-100 for 5 min and blocked with 5% goat serum for 60 min. Staining was performed using primary antibodies: anti-ErbB2 (Proteintech,18299-1-AP,1:200dilution), anti-ErbB3 (CellSignalingTechnology,12708,1:100dilution), LAMP1 (Proteintech; CL647-65050, 1:500 dilution ), V5 (Elabscience; E-AB-20010;1:1000 dilution).

Techniques: Stability, Expressing, Activation Assay, Analysis, Quantitative RT-PCR, Plasmid Preparation, Over Expression, Transfection, Western Blot, Confocal, Microscopy, Imaging