Journal: bioRxiv

Article Title: Genome-wide CRISPR screens identify DNA repair and R-loop suppression as regulators of the cellular sensitivity to environmentally relevant Bisphenol A exposure

doi: 10.64898/2026.04.13.718249

Figure Lengend Snippet: ( A, B ) DDX21 immunofluorescence showing that treatment with 0.5 μM BPA for 72 hours increases DDX21 foci formation in in RPE1 ( A ) and HeLa ( B ) cells. At least 105 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 and 20 µm are shown. ( C ) Western blot showing that HeLa and RPE1 cells do not express the estrogen receptor (ER). ER-expressing MCF7 cells used as a positive control. ( D ) Proximity ligation assay showing that treatment with 0.5 μM BPA for 72 hours or 0.5 μM Cisplatin for 24 hours increases the S9.6-dsDNA co-localization in HeLa cells. At least 101 cells were quantified for each condition. The mean value is represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). ( E ) Representative PLA experiment showing an increase in the number of S9.6-dsDNA foci in sgDDX21 cells, as compared to control HeLa cells, after 0.5 μM BPA for 72 hours. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired). Representative micrographs, with scale bars representing 10 µm are shown. ( F ) The means of 3 independent S9.6-dsDNA PLA experiments showing an increase in the mean number of foci in sgDDX21 cells, normalized to control HeLa cells, after 0.5 μM BPA for 72 hours across three independent replicates. The mean values are represented on the graphs, and asterisks indicate statistical significance (t-test two-tailed, unpaired).

Article Snippet: Antibodies used were: S9.6 (Kerafast ENH001), dsDNA (Novus Biologicals NBP3-07302).

Techniques: Immunofluorescence, Two Tailed Test, Western Blot, Expressing, Positive Control, Proximity Ligation Assay, Control