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Analysis of MHC and Desmin protein levels during C2C12 myoblast differentiation. Western blot analysis of C2C12 cells cultured on standard (Std) and LS surfaces at different stages of differentiation. Representative immunoblot for (a) MHC and Tubulin, and (c) Desmin and GAPDH. Densitometric analysis of (b) MHC and (d) Desmin protein levels. Data are presented as mean ± SD with individual data points ( n = 3). Statistical significance was assessed using Student's t ‐test ( * p < 0.05; ** p < 0.01; *** p < 0.001 vs. Standard at each time point).

Journal: Advanced Science

Article Title: Ultrafast Micrometric Laser Structuring of Polymer Surfaces for Highly Organized Skeletal Muscle Tissue Modeling

doi: 10.1002/advs.78129

Figure Lengend Snippet: Analysis of MHC and Desmin protein levels during C2C12 myoblast differentiation. Western blot analysis of C2C12 cells cultured on standard (Std) and LS surfaces at different stages of differentiation. Representative immunoblot for (a) MHC and Tubulin, and (c) Desmin and GAPDH. Densitometric analysis of (b) MHC and (d) Desmin protein levels. Data are presented as mean ± SD with individual data points ( n = 3). Statistical significance was assessed using Student's t ‐test ( * p < 0.05; ** p < 0.01; *** p < 0.001 vs. Standard at each time point).

Article Snippet: Membranes were blocked with 5% non‐fat dry milk in Tris‐buffered saline containing 0.05% Tween (TBS‐Tween) and then incubated overnight at 4°C with primary antibodies against Myosin Heavy Chain (MHC; 1:1000, Bio‐Techne MAB4470, monoclonal, clone MF20), Desmin (1:3000, Abclonal A3736, monoclonal, clone ARC0235), GAPDH (1:40000, Sigma MAB374, monoclonal, clone 6C5) and Tubulin (1:4000, Sigma T6199, monoclonal, clone DM1A).

Techniques: Analysis, Western Blot, Cell Culture