Journal: World Journal of Surgical Oncology

Article Title: The FGD5-AS1/miR-142-5p/CDK5 axis promotes ESCC progression by regulating mitochondrial fission and mitophagy: insights from integrative bioinformatics and experimental analyses

doi: 10.1186/s12957-026-04338-y

Figure Lengend Snippet: CDK5 as a target of miR-142-5p and its positive correlation with FGD5-AS1 expression. A qRT-PCR analysis of CDK5 expression levels in ESCC tissues and Adjacent tissue samples. ** p < 0.01; B Pearson correlation analysis of CDK5 and miR-142-5p expression levels. C - D qRT-PCR and Western blot analysis of CDK5 mRNA and protein expression levels in cells overexpressing or knocking down miR-142-5p. ** p < 0.01 vs. NC mimics; # p < 0.05, ## p < 0.01; E Predicted target sequences of CDK5 and miR-142-5p. F Dual-luciferase reporter assay validating the interaction between CDK5 and miR-142-5p. ** p < 0.01; G Pearson correlation analysis of FGD5-AS1 and CDK5 expression levels. H - I qRT-PCR and Western blot analysis of CDK5 expression levels in cells overexpressing or knocking down FGD5-AS1. * p < 0.05, ** p < 0.01

Article Snippet: After transfer, 5% non-fat milk was used to block the membranes at room temperature for 1 h. Overnight incubation at 4 °C was performed with primary antibodies against MFN1 (A21293), MFN2 (A19678), Drp1 (A2705), BECN1 (A21191), P62 (A19700), PINK1 (A25301), Parkin (A0968), CDK5 (A2689) (all 1:1000; ABclonal, China), p-Drp1 (3455), LC3-II (3868), LC3-I (4599) (all 1:1000; CST, USA), and β-actin (1:50000, AC026, ABclonal, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Luciferase, Reporter Assay

Journal: World Journal of Surgical Oncology

Article Title: The FGD5-AS1/miR-142-5p/CDK5 axis promotes ESCC progression by regulating mitochondrial fission and mitophagy: insights from integrative bioinformatics and experimental analyses

doi: 10.1186/s12957-026-04338-y

Figure Lengend Snippet: The FGD5-AS1/miR-142-5p/CDK5 axis influences ESCC progression. A Western blot analysis of CDK5 protein levels in shNC, sh-FGD5-AS1, sh-FGD5-AS1 + miR-142-5p inhibitor, and sh-FGD5-AS1 + OE-CDK5 groups of KYSE150 cells. B MTT assay of cell viability in the four groups. C TUNEL assay of apoptosis levels in the four groups. D Transwell assay of invasion capabilities in the four groups. E Scratch assay of migration capabilities in the four groups. F JC-1 assay of mitochondrial membrane potential in the four groups. G ATP assay of ATP levels in the four groups. H Western blot analysis of MFN1, MFN2, Drp1, and p-Drp1 protein levels in the four groups. I Western blot analysis of LC3-II, LC3-I, BECN1, P62, PINK1, and Parkin protein levels in the four groups. ** p < 0.01 vs. Control, ; ## p < 0.01 vs. sh-FGD5-AS1

Article Snippet: After transfer, 5% non-fat milk was used to block the membranes at room temperature for 1 h. Overnight incubation at 4 °C was performed with primary antibodies against MFN1 (A21293), MFN2 (A19678), Drp1 (A2705), BECN1 (A21191), P62 (A19700), PINK1 (A25301), Parkin (A0968), CDK5 (A2689) (all 1:1000; ABclonal, China), p-Drp1 (3455), LC3-II (3868), LC3-I (4599) (all 1:1000; CST, USA), and β-actin (1:50000, AC026, ABclonal, China).

Techniques: Western Blot, MTT Assay, TUNEL Assay, Transwell Assay, Wound Healing Assay, Migration, Membrane, ATP Assay, Control

Journal: Frontiers in Pharmacology

Article Title: Salidroside derivative SHPL-49 enhances synaptic remodeling in BCCAO rats via the CDK5/p35/p25 signaling pathway

doi: 10.3389/fphar.2026.1727177

Figure Lengend Snippet: SHPL-49 promotes the expression of CDK5 at both mRNA and protein levels. (A,B) Correlation analysis among samples. (C,D) Differential expression analysis between experimental groups. (E) Venn diagram showing the overlap of differentially expressed genes. (F) GO enrichment analysis of differentially expressed genes. (G–J) qRT-PCR validation of selected differentially expressed genes in primary neurons. (K–N) qRT-PCR validation of differentially expressed genes in rat brain tissue. (O) Representative Western blot images of CDK5 expression in primary neurons. (P) Quantitative analysis of CDK5 protein levels in primary neurons. (Q) Representative Western blot images of CDK5 expression in rat brain tissue. (R) Quantitative analysis of CDK5 protein levels in rat brain tissue. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, OGD/R group vs. Control group, Model group vs. Sham group compared with the Control group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group vs. OGD/R group. n = 3 for mRNA analysis in primary neurons and rat brain tissue. n = 6 for protein analysis in primary neurons and rat brain tissue.

Article Snippet: Membranes were blocked with 5% non-fat milk (Beyotime, China) in Tris-buffered saline containing 0.1% Tween-20 (TBST) and incubated overnight at 4 °C with the following primary antibodies: SYP (1:1,000, CST, United States), SYN1 (1:1,000, Boster, China), PSD95 (1:1,000, ABCAM, United States), CDK5 (1:1,000, Boster, China), p35/25 (1:1,000, CST, United States), p-PSD95 (1:1,000, CST, United States).

Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR, Biomarker Discovery, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Salidroside derivative SHPL-49 enhances synaptic remodeling in BCCAO rats via the CDK5/p35/p25 signaling pathway

doi: 10.3389/fphar.2026.1727177

Figure Lengend Snippet: SHPL-49 inhibits the proteolytic conversion of p35 to p25. (A) Representative Western blot images showing p35 and p25 expression in primary neurons. (B) Quantitative analysis of p35 protein lev-els in primary neurons. (C) Quantitative analysis of p25 protein levels in primary neurons. (D) Representative Western blot images of p35 and p25 in rat brain tissue. (E) Quantitative analysis of p35 protein levels in rat brain tissue. (F) Quantitative analysis of p25 protein levels in rat brain tis-sue. (G) Representative Western blot images showing the expression levels of CDK5, p35, p25, and p-PSD95/PSD95 ratio in primary neurons following OGD/R treatment and co-treatment with SHPL-49 and CDK5 inhibitor Roscovitine. (H) Quantitative analysis of CDK5 protein levels in primary neurons under the same experimental conditions. (I) Quantitative analysis of p35 protein levels in primary neurons under the same experimental conditions. (J) Quantitative analysis of p25 protein levels in primary neurons under the same experimental conditions. (K) Quantitative analysis of p-PSD95/PSD95 ratio in primary neurons under the same experimental conditions. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001, OGD/R group vs. Control group, Model group vs. Sham group, # P < 0.05, ## P < 0.01, ### P < 0.001, SHPL-49 group or PD151746 group vs. OGD/R group, SHPL-49 group or SAL group vs. Model group n = 6 per group.

Article Snippet: Membranes were blocked with 5% non-fat milk (Beyotime, China) in Tris-buffered saline containing 0.1% Tween-20 (TBST) and incubated overnight at 4 °C with the following primary antibodies: SYP (1:1,000, CST, United States), SYN1 (1:1,000, Boster, China), PSD95 (1:1,000, ABCAM, United States), CDK5 (1:1,000, Boster, China), p35/25 (1:1,000, CST, United States), p-PSD95 (1:1,000, CST, United States).

Techniques: Western Blot, Expressing, Control