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Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in <t>thapsigargin-treated</t> <t>AML-12</t> cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.
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Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in <t>thapsigargin-treated</t> <t>AML-12</t> cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.
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Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in thapsigargin-treated AML-12 cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.

Journal: World Journal of Stem Cells

Article Title: GATA2 deficiency exacerbates chronic liver injury via disrupting hepatocyte death-regeneration balance: Clinical, histopathological, and molecular evidence

doi: 10.4252/wjsc.v18.i2.112940

Figure Lengend Snippet: Elevated GATA2 protein expression in hepatocytes under endoplasmic reticulum stress and oxidative stress. A: Cell viability assessed by Cell Counting Kit-8 assay in thapsigargin-treated AML-12 cells; B: Semi-quantitative analysis of thapsigargin-induced GATA2 protein expression by western blotting (representative blots shown); C and E: Cell viability measured by Cell Counting Kit-8 assay in AML-12 (C) and HepG2 cells (E) treated with H 2 O 2 ; D and F: Semi-quantitative analysis of H 2 O 2 -induced GATA2 protein expression by western blotting in AML-12 (D) and HepG2 cells (F) (representative blots shown). b P < 0.01 vs control group. HSP60: Heat shock protein 60.

Article Snippet: Human hepatocellular carcinoma HepG2 cells and mouse hepatocyte AML-12 cells were purchased from the American Type Culture Collection and authenticated via short tandem repeat profiling.

Techniques: Expressing, Cell Counting, Western Blot, Control