Journal: Stem Cell Reports

Article Title: Diminished RELB impairs osteogenesis in mature periodontal ligament stem cells

doi: 10.1016/j.stemcr.2026.103088

Figure Lengend Snippet:

Article Snippet: After 7 days of osteogenic induction, the cells were fixed in 4% paraformaldehyde for 30 min and then stained using a BCIP/NBT ALP color development kit (Beyotime, Shanghai, China, C3206) for 30 min at room temperature.

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Natural polysaccharide-engineered POP@ZIF-67 nanoparticles coordinate antibacterial activity, macrophage polarization, and osteogenesis for chronic osteomyelitis therapy

doi: 10.3389/fbioe.2026.1935416

Figure Lengend Snippet: Effect of POP@ZIF-67 on osteogenic differentiation of MC3T3-E1 cells under infection stress conditions. (A) CCK-8 cell viability assay of MC3T3-E1 cells. (B) Quantitative analysis of ALP-positive area. (C) Representative image of ALP staining. (D) Quantitative analysis of ARS-positive area. (E) Representative image of ARS staining. (F) Relative expression levels of BMP2 mRNA. (G) Relative expression levels of Smad1 mRNA. Data are presented as mean ± SD, n = 3. ns, not significant; **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: For alkaline phosphatase (ALP) staining, cells were fixed after early osteogenic induction and stained with an ALP staining kit (Beyotime, Shanghai, China).

Techniques: Infection, CCK-8 Assay, Cell Viability Assay, Analysis, Staining, Expressing