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ATCC agar well diffusion assay against escherichia coli atcc 25922
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Nissui Pharmaceutical compactdrytm tcr agar diffusion disks
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Kuang Lung Shing agar diffusion assay
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Liofilchem agar gradient diffusion methodology
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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ATCC agar diffusion method against s aureus
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Thermo Fisher agar diffusion assays
Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).
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Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: Antibacterial activities of Chitligsan samples, prepared with distilled water and 0.1 M citric acid, against E. coli (ATCC 25922), S. aureus (ATCC 29213), and P. aeruginosa (ATCC 27853). Supernatant: supernatant of Chitligsan suspension, blank: distilled water (DI), ciprofloxacin (CIP) (5 µg mL −1 ), 1: Chitligsan DI extract (1 mg mL −1 ), 2: 0.1 M citric acid (control), 3: Chitligsan 0.1 M citric acid extract (1 mg mL −1 ).

Article Snippet: Antimicrobial activity of Chitligsan was evaluated using agar well diffusion assay against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213.

Techniques: Suspension, Control

SEM micrographs illustrating the morphological changes of S. aureus (A–C) and E. coli (D–F) upon exposure to Chitligsan or citric acid. (A and D) Untreated control cells; (B and E) cells treated with 0.1 M citric acid; (C and F) cells treated with Chitligsan (1 mg mL −1 ) and 0.1 M citric acid.

Journal: RSC Advances

Article Title: Sahara Desert sand “Chitligsan”: characterisation and assessment of antibacterial activity and cytotoxicity

doi: 10.1039/d5ra04969e

Figure Lengend Snippet: SEM micrographs illustrating the morphological changes of S. aureus (A–C) and E. coli (D–F) upon exposure to Chitligsan or citric acid. (A and D) Untreated control cells; (B and E) cells treated with 0.1 M citric acid; (C and F) cells treated with Chitligsan (1 mg mL −1 ) and 0.1 M citric acid.

Article Snippet: Antimicrobial activity of Chitligsan was evaluated using agar well diffusion assay against Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213.

Techniques: Control