Journal: bioRxiv

Article Title: Radiotherapy-induced vascular dysfunction alters neutrophil phenotype to promote tumor cell colonization of mammary adipose tissue

doi: 10.64898/2026.10.03.756445

Figure Lengend Snippet: a. Experimental timeline for CD8 + T cell redution experiments. Female BALB/c mice were inoculated with 4T1 luc cells and treated with 500µg of CD8 or IgG control antibodies at the time of inoculation and every 5 days therafter. When tumors were 100mm 3 , the contralateral MFP was irradiate to 20 Gy and MFPs were collected for downstream analysis at 10 days post-RT. Representative images (b) and quantification (c) of Ly6G + cells in MFPs of BALB/c mice. n=5 mice per condition. **p<0.01 and ***p<0.001 by ANOVA with Sidak’s test. d. Quantification of CD45 + CD11b + Ly6C mid Ly6G + SSC hi neutrophils in MFPs at 10d post-RT analyzed via flow cytometry. n=8-12 mice per condition. p-values determined by ANOVA with Sidak’s test. e. Experimental timeline for Nu/Nu experiments with details as noted above except antibody treatments. f. Quantification of CD45 + CD11b + Ly6C mid Ly6G + neutrophils in MFPs of Nu/Nu mice at 10d post-RT analyzed via flow cytometry. n=5 mice per condition. p-value determined by t-test. (g) Significantly enriched pathways related to tissue remodeling in irradiated CD8 mice determined by Reactome analysis of MFP bulk RNA-seq data. n=5 mice per condition. h. Representative images (b) and quantification (c) of percent CD31 + area in MFPs of BALB/c mice. n=5 mice per condition. *p<0.05 and ***p<0.001 by ANOVA with Sidak’s test. j. Experimental timeline for neutrophil depletion model. Nu/Nu mice were inoculated and treated as in (e) with additional i.p. injections with Ly6G or IgG control antibodies at the time of inoculation and every other day thereafter. k. Fold change of lucb expression relative to Actb in 20 Gy vs. 0 Gy contralateral MFPs via RT-qPCR. n=8-12 mice per condition. p-value determined by Mann-Whitney test.

Article Snippet: Luciferase-labeled 4T1 mouse mammary carcinoma cells (4T1 luc ) were obtained from ATCC and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S).

Techniques: Control, Irradiation, Analysis, Flow Cytometry, Tissue, RNA Sequencing, Expressing, Quantitative RT-PCR, MANN-WHITNEY, Protein-Protein interactions, Significance Assay

Journal: bioRxiv

Article Title: Radiotherapy-induced vascular dysfunction alters neutrophil phenotype to promote tumor cell colonization of mammary adipose tissue

doi: 10.64898/2026.10.03.756445

Figure Lengend Snippet: a. Representative images of CD31-VEGF costaining of MFPs in Nu/Nu neutrophil depletion model. Scale bars are 50µm. Coresponding quantification of CD31 + vessels per mm 2 (b) and integrated density of VEGF localized with CD31 + vessels (c). n=4-5 mice per condition. *p<0.05 and **p<0.01 determined by two-way ANOVA with Sidak’s test. Quantification (d) and (e) representative images of F4/80 + cells per mm 2 in MFPs from Nu/Nu neutrophil depletion model. n=3-5 mice per condition. ***p<0.001 determined by two-way ANOVA with Sidak’s test. f. Gene expression of select genes related to a pathologically-activated PMN-MDSC phenotype from bulk RNA-seq analysis of MFPs of IgG and CD8 mice in 20 vs. 0 Gy MFPs. n=5 mice per condition. g. Representative images of neutrophil extracellular traps (NETs) via localization of DAPI, MPO, and CitH3 in MFPs of Nu/Nu mice bearing contralateral 4T1 luc tumors. Arrows indicate localization of all three stains. Quantification (h) and representative images (i) of S100A9 + cells in MFPs from Nu/Nu-4T1 luc model. n=2-5 mice per condition j. Flow cytometry analysis of expression of key PMN-MDSC markers shown as percent of neutrophils. Neutrophils were defined as live CD45 + Ly6C mid Ly6G + cells in MFP, spleen, blood, and bone marrow from Nu/Nu 4T1 luc model. n=5 mice per condition. **p<0.01 and ***p<0.001 by two-way ANOVA with organ as the repeated measures factor followed by Sidak’s test. All data show mean±SD. All error bars are 50µm.

Article Snippet: Luciferase-labeled 4T1 mouse mammary carcinoma cells (4T1 luc ) were obtained from ATCC and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S).

Techniques: Gene Expression, RNA Sequencing, Analysis, Flow Cytometry, Expressing

Journal: bioRxiv

Article Title: Radiotherapy-induced vascular dysfunction alters neutrophil phenotype to promote tumor cell colonization of mammary adipose tissue

doi: 10.64898/2026.10.03.756445

Figure Lengend Snippet: a. Flow cytometry analysis of CD45 + CD11b + Ly6C mid Ly6G + cells in Nu/Nu-4T1 luc neutorphil depletion model without RT. n=6-9 mice per condition. ***p<0.001 by Mann-Whitney test. b. Mouse metrics over time in Nu/Nu-4T1 luc neutrophil depletion model with RT. n=5 mice per condition. Data show mean±SD.

Article Snippet: Luciferase-labeled 4T1 mouse mammary carcinoma cells (4T1 luc ) were obtained from ATCC and maintained in RPMI-1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (P/S).

Techniques: Flow Cytometry, Analysis, MANN-WHITNEY

Journal: Advanced Science

Article Title: Self‐Assembling Peptide‐Adjuvant Conjugate (SaPAC) Platform for Precision Cancer Immunotherapy

doi: 10.1002/advs.77632

Figure Lengend Snippet: Synergistic antitumor efficacy of trivalent SaPAC‐4T1 vaccination combined with anti‐PD‐1 checkpoint blockade in orthotopic 4T1 TNBC. (A) Treatment timeline. (B) Representative endpoint tumors (day 16; scale bar, 10 mm). (C) Longitudinal tumor volumes ( n = 6–8 mice/group). (D) Terminal tumor volumes on day 15. (E,F) Individual tumor growth trajectories. Data are the mean ± SEM; significance was assessed by unpaired two‐tailed Student's t‐test ( * p <0.05, ** p <0.01, *** p <0.001 vs. mock + anti‐PD‐1).

Article Snippet: The DC2.4 cell line, mouse bladder cancer cell line MB49, mouse melanoma cell line B16F10, and the mouse breast cancer cell line 4T1 were purchased from ATCC.

Techniques: Two Tailed Test

Journal: Advanced Science

Article Title: Self‐Assembling Peptide‐Adjuvant Conjugate (SaPAC) Platform for Precision Cancer Immunotherapy

doi: 10.1002/advs.77632

Figure Lengend Snippet: Peptides, peptide‐adjuvant conjugates, and fluorescently labeled peptides used in this study. The antigen epitope, linker/self‐assembly residues, and 1V209‐conjugated lysines (K * ) are indicated in the sequence column.

Article Snippet: The DC2.4 cell line, mouse bladder cancer cell line MB49, mouse melanoma cell line B16F10, and the mouse breast cancer cell line 4T1 were purchased from ATCC.

Techniques: Labeling, Sequencing, Conjugation Assay