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Figure Lengend Snippet: Number of 16S reads attributed to three of the common pathogens known to cause skin infectious diseases.

Article Snippet: Library preparation targeted the V1-V2 variable regions of the 16S rRNA gene (ca. 300 bp), using the quick 16S Next Generation library preparation kits (Zymo research, USA) according to manufacturer’s instructions (described in the Supporting Information file SI1 and S1 – S10 Fig. ).

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Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Isolation and characterization of multidrug-resistant Proteus mirabilis from diarrheic sika deer fawns in Northeast China

doi: 10.3389/fcimb.2026.1866786

Figure Lengend Snippet: Neighbor-Joining phylogenetic tree based on 1,341-bp 16S rRNA gene sequences of 75 Proteus mirabilis isolates and reference strains. The tree was constructed in MEGA 12 using the Neighbor-Joining method with 1,000 bootstrap replicates. Bootstrap values ≥50% are shown at the nodes. GenBank accession numbers, where available, are shown in parentheses. Superscript T indicates a type strain. P. mirabilis ATCC 29906 T was included as the type strain, whereas P. mirabilis ATCC 12453 was included as the laboratory reference strain. Type strains of closely related Proteus species were included for species-level comparison. The scale bar represents 0.002 substitutions per site.

Article Snippet: The 16S rRNA phylogenetic tree ( ) showed that all 75 study isolates were positioned within the P. mirabilis lineage containing the type strain ATCC 29906 T , the laboratory reference strain ATCC 12453, and additional P. mirabilis reference sequences, and were separated from the type strains of closely related Proteus species included for comparison.

Techniques: Construct, Comparison