Journal: Cell Reports Medicine

Article Title: CD97 maintains tumorigenicity of glioblastoma stem cells via mTORC2 signaling and is targeted by CAR Th9 cells

doi: 10.1016/j.xcrm.2024.101844

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Article Snippet: GSCs were plated at a density of 6 × 10 5 cells/well densities in 60 mm culture dishes and incubated with DMEM/F-12 supplemented with B27, EGF (10 ng/mL), and bFGF (5 ng/mL) containing Torin1 (50 and 100 nM, for 48 h; SelleckChem, Houston, TX, USA), AKT inhibitor IV (10 and 20 μM, for 24 h; Cruz Credit, Santa Cruz, CA, USA), Rapamycin (5 and 10 nM, for 24 h; SelleckChem), and JR-AB2-011 (10 and 20 μM, for 48 h; MedChemExpress, Monmouth Junction, NJ, USA).

Techniques: Produced, Virus, Plasmid Preparation, Recombinant, Purification, Cell Culture, Cell Isolation, Reporter Gene Assay, cDNA Synthesis, Apoptosis Assay, Cytotoxicity Assay, Gene Expression, shRNA, Sequencing, Amplification, Software, Microscopy, Western Blot