Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: Scatter plot of the gene differential expression in PC-2 cells induced by RPM. Notes: The Cy3 (RPM treated group) and Cy5 (untreated control) channel intensities from the two-color DNA microarray experiments were shown in the scatter plot. The variables appear in a linear relationship, and the linear correlation between them is 0.935. The red plots represented upregulated genes, and the green plots represented downregulated genes. Abbreviation: RPM, rapamycin.

Article Snippet: We used an Agilent Whole Human Genome Microarray chip to assess the expression profiles in RPM-treated cells.

Techniques: Expressing, Microarray

Journal: Drug Design, Development and Therapy

Article Title: Targeted inhibition of mammalian target of rapamycin (mTOR) enhances radiosensitivity in pancreatic carcinoma cells

doi: 10.2147/DDDT.S42390

Figure Lengend Snippet: ( A ) RT-PCR analysis for the DNA-damage repair and transcription genes after the treatment with RPM; ( B ) Difference between the cDNA microarray and RT-PCR in DNA-damage repair and transcription genes. Notes: The genes examined here are: (1) DDB1(NM_001923); (2) RAD51 (NM_002876); (3) XRCC5 (NM_021141); (4) PCNA (NM_002592); and (5) ABCC4 (NM_005845). The values represent the mean ± SD of the data from three independent experiments. Abbreviations: RPM, rapamycin; RT-PCR, reverse transcription polymerase chain reaction; SD, standard deviation.

Article Snippet: We used an Agilent Whole Human Genome Microarray chip to assess the expression profiles in RPM-treated cells.

Techniques: Reverse Transcription Polymerase Chain Reaction, Microarray, Standard Deviation

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Characteristics of the samples used for microarray analysis

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Microarray analyses of gene expression of osteoarthritis and non-osteoarthritis of subchondral bone and qRT-PCR validation in osteoarthritis knee subchondral bone. (A) Differentially expressed genes were classified based on their expression levels with a minimum of twofold, threefold, fourfold, sixfold, and eightfold changes. (B) Unsupervised hierarchical clustering of osteoarthritis (OA) and non-OA samples was performed for genes whose differential expression exceeded twofold (972 genes). Distances between samples were detected with a Euclidean algorithm and clustered with a centroid linkage method. The OA medial tibial (MT) samples (gray) were clustered together and clearly separated from the OA lateral tibial (LT) (brown) and non-OA samples. Non-OA LT samples (N-LT, red) and non-OA MT samples (N-MT, blue) were clustered together with OA LT samples. (C) Eighty-five genes (plus GAPDH ) were selected for quantitative reverse-transcription polymerase chain reaction (qRT-PCR) validation on a separate group of nine OA subchondral bone specimens. The results of qRT-PCR were strongly consistent with those of microarray analysis ( R 2 = 0.5764, P <0.0001). FC, fold-change.

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray, Expressing, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Top ten upregulated and downregulated genes comparing lateral tibial bone (below intact cartilage) with medial tibial bone (below lesioned cartilage)

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray, Expressing, Binding Assay

Journal: Arthritis Research & Therapy

Article Title: Genome-wide expression profiles of subchondral bone in osteoarthritis

doi: 10.1186/ar4380

Figure Lengend Snippet: Genes representative of gene clusters associated with osteoarthritis

Article Snippet: Cyanine 3-labeled cRNA was then purified and hybridized to Agilent whole human genome 44 k microarray chips (Agilent Technologies).

Techniques: Microarray

Journal: BMC Musculoskeletal Disorders

Article Title: Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease

doi: 10.1186/1471-2474-13-130

Figure Lengend Snippet: Characteristics of KBD and control sample pairs

Article Snippet: Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data.

Techniques: Microarray

Journal: BMC Musculoskeletal Disorders

Article Title: Expression profile analysis of mycotoxin-related genes in cartilage with endemic osteochondropathy kashin-beck disease

doi: 10.1186/1471-2474-13-130

Figure Lengend Snippet: Histogram showing the expression values of the selected 8 genes measured by microarray and qRT-PCR.

Article Snippet: Total RNA was isolated from cartilage samples, following by being amplified, labeled and hybridized to Agilent human whole genome microarray chip. qRT-PCR was conducted to validate the microarray data.

Techniques: Expressing, Microarray, Quantitative RT-PCR